Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   RCG24_RS01350 Genome accession   NZ_CP133263
Coordinates   259292..261655 (+) Length   787 a.a.
NCBI ID   WP_308078132.1    Uniprot ID   -
Organism   Neobacillus sp. OS1-32     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 254292..266655
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCG24_RS01335 (RCG24_01335) spoVB 254577..256130 (-) 1554 WP_308078131.1 stage V sporulation protein B -
  RCG24_RS01340 (RCG24_01340) - 256253..256552 (+) 300 WP_306792899.1 post-transcriptional regulator -
  RCG24_RS01345 (RCG24_01345) secDF 256704..258968 (+) 2265 WP_202652357.1 protein translocase subunit SecDF -
  RCG24_RS01350 (RCG24_01350) recJ 259292..261655 (+) 2364 WP_308078132.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  RCG24_RS01355 (RCG24_01355) - 261646..262158 (+) 513 WP_202652360.1 adenine phosphoribosyltransferase -
  RCG24_RS01360 (RCG24_01360) - 262396..264591 (+) 2196 WP_308078133.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  RCG24_RS01365 (RCG24_01365) dtd 264604..265047 (+) 444 WP_202652364.1 D-aminoacyl-tRNA deacylase -
  RCG24_RS01370 (RCG24_01370) - 265205..265381 (+) 177 WP_202652366.1 hypothetical protein -

Sequence

Protein


Download         Length: 787 a.a.        Molecular weight: 87940.74 Da        Isoelectric Point: 5.6173

>NTDB_id=872884 RCG24_RS01350 WP_308078132.1 259292..261655(+) (recJ) [Neobacillus sp. OS1-32]
MLKPKTRWVVRHSDHQLAESLANELNITPLVATLLVNRGFDTASSARFFLFGKEEFYDPFLFNGMETAVKRIRAAIDQNE
PILIFGDYDADGVSSTSVLMLTLRDLGANVQFYIPNRFSEGYGPNEPAFRHAAELGIKLIITVDTGIAAVHEAEVAKQLG
IDLIITDHHEPGPVLPHALAIIHPKLADSTYPFRELAGVGVAFKLAHALYGRLPEHLLEIAVIGTIADLVSLTGENRLIA
KNGLQKLKETERIGLRAIFKTAGIDPLAINEETIGFSIAPRINAVGRLADADLAVQLLLTEDPVEAEELAAEMEALNKSR
QALVNTMTEEAIAEVETHYPIDSNKVLVIGKEGWNAGVIGIVASKLVEKYYRPAIVLSFDQEKGLAKGSARSIPGFDLFQ
NLSTLRDILPHFGGHPMAAGMTLRLDDVAELRQKLNQLAADVLTDDDFIPVTYLDNEVPLKEIDLQSLEEMRLLSPYGID
NPKPKVLINNVGITSLRKIGSDQNHLKITVNDHDAHLDGVGFGLGAVVDQISPAAKISLIGELSINEWNNIRKPQIFVQD
IAVRSWQLFDFRGQKHLGSIPVTVPEENRKLIVFNKHHAEKINKHLGIEPVCIEDVESAKAFDGYHANIALVDFPPSKEI
LIQLLKGKQPARIYAYFYKASSDFFSTIPTRDHFKWFYAFLLKNNPIDLNRYGDDIAKLRGWSRSTINFMTKVFSELNFV
TINNGFITLNKQPQKRDLTDSRTFQQKQAQSELERDLLFSSFQELKQWFTNIIEESVEIEEAIKQWI

Nucleotide


Download         Length: 2364 bp        

>NTDB_id=872884 RCG24_RS01350 WP_308078132.1 259292..261655(+) (recJ) [Neobacillus sp. OS1-32]
ATGTTAAAACCGAAAACAAGATGGGTTGTCCGTCATTCCGATCATCAACTGGCTGAGTCGCTTGCCAATGAATTAAACAT
CACCCCTCTTGTTGCAACATTGTTAGTTAACCGTGGATTTGATACAGCTTCCTCCGCACGGTTTTTTTTATTTGGAAAAG
AAGAGTTTTATGATCCATTTTTATTTAACGGAATGGAGACAGCTGTAAAGCGTATTCGTGCTGCCATTGATCAGAATGAA
CCGATTCTCATTTTTGGTGACTACGATGCAGACGGGGTCAGCAGTACTTCCGTGTTAATGCTTACACTTCGTGATTTAGG
GGCCAATGTCCAATTCTATATTCCAAATCGTTTCTCAGAAGGGTATGGTCCGAACGAACCCGCCTTTCGCCATGCAGCGG
AGCTTGGAATTAAACTGATTATTACCGTTGACACTGGCATAGCAGCGGTTCACGAGGCGGAAGTGGCCAAGCAGTTGGGG
ATTGACCTGATCATTACGGACCACCATGAGCCTGGGCCGGTATTACCACATGCGCTGGCAATCATTCACCCGAAGCTTGC
TGACAGCACCTATCCGTTTCGCGAGTTGGCCGGGGTAGGAGTAGCCTTTAAATTAGCCCATGCTCTTTATGGACGCCTTC
CTGAGCATTTATTGGAAATTGCCGTCATCGGGACGATTGCTGACTTGGTTTCCTTAACAGGTGAAAATCGCTTAATCGCT
AAAAATGGTTTGCAGAAATTGAAGGAAACGGAAAGAATCGGGCTAAGAGCGATTTTTAAAACTGCGGGGATCGATCCGCT
GGCGATTAATGAAGAGACGATTGGATTTTCAATCGCACCGCGAATTAACGCAGTTGGAAGGCTGGCGGATGCCGATTTAG
CTGTTCAACTTCTCTTAACTGAGGACCCTGTTGAGGCAGAGGAGCTTGCAGCTGAGATGGAAGCCTTGAATAAATCACGG
CAAGCACTTGTCAATACGATGACGGAAGAAGCGATTGCCGAGGTTGAAACCCATTACCCGATTGACAGCAATAAGGTTCT
GGTGATTGGGAAAGAAGGCTGGAATGCCGGGGTAATTGGCATTGTTGCTTCCAAACTGGTGGAAAAATATTATCGTCCGG
CGATCGTCCTCAGTTTTGATCAAGAAAAAGGCTTAGCGAAAGGATCAGCACGAAGTATTCCCGGGTTTGATCTATTTCAA
AATTTATCGACTTTACGAGATATTCTACCTCATTTTGGCGGGCATCCAATGGCAGCCGGGATGACCTTGAGGCTTGACGA
TGTGGCGGAACTGCGTCAGAAATTGAATCAGTTGGCTGCTGATGTACTCACTGATGATGATTTTATCCCGGTAACGTATT
TGGATAATGAAGTACCTTTGAAAGAAATTGATTTACAATCGCTGGAAGAAATGAGGTTACTCTCTCCCTACGGAATAGAC
AATCCTAAACCAAAGGTGTTAATTAATAATGTGGGGATTACTAGCCTCAGAAAAATTGGAAGTGACCAAAATCATTTAAA
GATTACCGTTAATGATCATGATGCCCATCTTGATGGAGTAGGGTTTGGTCTTGGAGCGGTAGTCGATCAAATCTCACCTG
CTGCAAAAATTTCATTAATTGGTGAATTATCGATTAATGAATGGAACAATATACGTAAACCGCAAATTTTTGTCCAAGAT
ATCGCTGTTCGCTCATGGCAATTGTTCGATTTTCGCGGACAAAAGCATCTGGGGAGTATTCCGGTGACTGTACCGGAGGA
AAATCGAAAACTCATTGTTTTCAACAAGCATCATGCGGAAAAGATAAACAAGCATCTTGGAATTGAGCCGGTTTGTATTG
AAGATGTGGAATCGGCAAAAGCATTTGATGGCTATCATGCCAATATTGCCTTAGTCGATTTCCCGCCTTCAAAGGAAATT
CTGATTCAATTATTGAAAGGGAAACAGCCAGCAAGAATATACGCATATTTCTATAAAGCTTCAAGTGACTTTTTCAGCAC
GATACCAACCCGTGATCATTTTAAATGGTTTTATGCCTTTCTTTTAAAAAATAATCCCATTGATCTTAACCGCTATGGAG
ATGACATAGCAAAATTAAGAGGCTGGTCTCGGTCAACAATCAACTTTATGACAAAGGTGTTTTCTGAACTGAATTTTGTT
ACAATAAACAATGGATTTATTACTTTAAATAAACAACCACAGAAGCGCGATTTAACTGACTCAAGAACATTTCAGCAAAA
GCAGGCCCAATCAGAACTTGAACGGGATTTATTATTTTCATCATTTCAAGAATTAAAACAATGGTTTACCAATATTATTG
AAGAGTCAGTTGAAATTGAGGAGGCTATTAAACAATGGATTTAA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(455-560)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(567-769)

DHH family (DHH)

(82-225)

DHHA1 domain (DHHA1)

(314-438)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

54.384

100

0.544