Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   K6K02_RS13135 Genome accession   NZ_AP024626
Coordinates   2522344..2522727 (-) Length   127 a.a.
NCBI ID   WP_003230168.1    Uniprot ID   -
Organism   Bacillus subtilis strain BEST3125     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2517344..2527727
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6K02_RS13095 (BsBEST3125_25100) sinI 2518278..2518451 (+) 174 WP_003230187.1 anti-repressor SinI Regulator
  K6K02_RS13100 (BsBEST3125_25110) sinR 2518485..2518820 (+) 336 WP_003226345.1 transcriptional regulator SinR Regulator
  K6K02_RS13105 (BsBEST3125_25120) tasA 2518913..2519698 (-) 786 WP_004398632.1 biofilm matrix protein TasA -
  K6K02_RS13110 (BsBEST3125_25130) sipW 2519762..2520334 (-) 573 WP_003246088.1 signal peptidase I SipW -
  K6K02_RS13115 (BsBEST3125_25140) tapA 2520318..2521079 (-) 762 WP_004399106.1 amyloid fiber anchoring/assembly protein TapA -
  K6K02_RS13120 (BsBEST3125_25150) yqzG 2521351..2521677 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  K6K02_RS13125 (BsBEST3125_25160) spoIITA 2521719..2521898 (-) 180 WP_003230176.1 YqzE family protein -
  K6K02_RS13130 (BsBEST3125_25170) comGG 2521969..2522343 (-) 375 WP_003230170.1 ComG operon protein ComGG Machinery gene
  K6K02_RS13135 (BsBEST3125_25180) comGF 2522344..2522727 (-) 384 WP_003230168.1 ComG operon protein ComGF Machinery gene
  K6K02_RS13140 (BsBEST3125_25190) comGE 2522753..2523100 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  K6K02_RS13145 (BsBEST3125_25200) comGD 2523084..2523515 (-) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  K6K02_RS13150 (BsBEST3125_25210) comGC 2523505..2523801 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  K6K02_RS13155 (BsBEST3125_25220) comGB 2523815..2524852 (-) 1038 WP_009967727.1 comG operon protein ComGB Machinery gene
  K6K02_RS13160 (BsBEST3125_25230) comGA 2524839..2525909 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  K6K02_RS13165 (BsBEST3125_25240) corA 2526321..2527274 (-) 954 WP_004399136.1 magnesium transporter CorA -

Sequence

Protein


Download         Length: 127 a.a.        Molecular weight: 14281.37 Da        Isoelectric Point: 5.8929

>NTDB_id=87209 K6K02_RS13135 WP_003230168.1 2522344..2522727(-) (comGF) [Bacillus subtilis strain BEST3125]
MLISGSLAAIIHLFLSRQQEHDGFTQQEWMISIEQMMNECKESQAVKTAEHGSVLICTNLSGQDIRFDIYHSMIRKRVDG
KGHVPILDHITAMKADIENGVVLLKIESEDQKVYQTAFPVYSYLGGG

Nucleotide


Download         Length: 384 bp        

>NTDB_id=87209 K6K02_RS13135 WP_003230168.1 2522344..2522727(-) (comGF) [Bacillus subtilis strain BEST3125]
TTGCTCATATCAGGATCGTTAGCTGCGATTATCCATCTGTTTTTGTCTCGACAGCAGGAACATGACGGTTTCACACAGCA
GGAATGGATGATTTCGATAGAACAGATGATGAATGAATGCAAGGAATCACAGGCAGTTAAGACAGCCGAGCATGGGAGCG
TGTTAATCTGCACCAATCTTTCCGGACAAGACATCCGTTTTGACATTTATCATTCAATGATAAGAAAAAGAGTGGATGGC
AAAGGGCATGTTCCGATTTTAGATCATATTACTGCCATGAAAGCTGATATTGAAAATGGTGTTGTTTTGCTGAAAATTGA
GAGTGAAGACCAAAAAGTGTATCAAACTGCTTTTCCAGTCTATTCGTATTTAGGAGGGGGGTGA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(16-114)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment