Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WHU44_RS10010 Genome accession   NZ_CP148319
Coordinates   1959973..1960440 (+) Length   155 a.a.
NCBI ID   WP_000381746.1    Uniprot ID   Q8X892
Organism   Escherichia coli strain Z1323HEC0494     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1954973..1965440
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHU44_RS09965 (WHU44_09965) rplP 1955083..1955493 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  WHU44_RS09970 (WHU44_09970) rpsC 1955506..1956207 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  WHU44_RS09975 (WHU44_09975) rplV 1956225..1956557 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  WHU44_RS09980 (WHU44_09980) rpsS 1956572..1956850 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  WHU44_RS09985 (WHU44_09985) rplB 1956867..1957688 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  WHU44_RS09990 (WHU44_09990) rplW 1957706..1958008 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  WHU44_RS09995 (WHU44_09995) rplD 1958005..1958610 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  WHU44_RS10000 (WHU44_10000) rplC 1958621..1959250 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  WHU44_RS10005 (WHU44_10005) rpsJ 1959283..1959594 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  WHU44_RS10010 (WHU44_10010) pilD 1959973..1960440 (+) 468 WP_000381746.1 A24 family peptidase Machinery gene
  WHU44_RS10015 (WHU44_10015) bfr 1960437..1960913 (-) 477 WP_000675516.1 bacterioferritin -
  WHU44_RS10020 (WHU44_10020) bfd 1960986..1961180 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  WHU44_RS10025 (WHU44_10025) tuf 1961363..1962547 (-) 1185 WP_000031783.1 elongation factor Tu -
  WHU44_RS10030 (WHU44_10030) fusA 1962618..1964732 (-) 2115 WP_000124700.1 elongation factor G -
  WHU44_RS10035 (WHU44_10035) rpsG 1964829..1965299 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17099.29 Da        Isoelectric Point: 8.1343

>NTDB_id=870070 WHU44_RS10010 WP_000381746.1 1959973..1960440(+) (pilD) [Escherichia coli strain Z1323HEC0494]
MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHSWAFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=870070 WHU44_RS10010 WP_000381746.1 1959973..1960440(+) (pilD) [Escherichia coli strain Z1323HEC0494]
ATGGACACTACCCTTCCCTTTCTAATTTTGTACGCTTGTTTATCAGCCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACGTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAAGTTTGTCATCCTGATGGTTTAGCCG
ATGCGTTATGGGGGGCAATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGCCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGCGCCTGGCATTCATGGGCCTTCTTACCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTCTGCTCATGAGAGGAAAAGAATCATTAAAAAACCCGCTGC
CTTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTATAGGCTGGGATAGTTTGCTGATAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(11-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8X892

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

43.478

89.032

0.387