Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RAB71_RS14790 Genome accession   NZ_CP132343
Coordinates   3505838..3506968 (-) Length   376 a.a.
NCBI ID   WP_010344137.1    Uniprot ID   -
Organism   Xanthomonas sacchari strain CFBP4641     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3500838..3511968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAB71_RS14770 (RAB71_14765) - 3500991..3501665 (-) 675 WP_010344133.1 YitT family protein -
  RAB71_RS14775 (RAB71_14770) - 3501849..3502448 (-) 600 WP_010344134.1 MFS transporter -
  RAB71_RS14780 (RAB71_14775) - 3502941..3504440 (-) 1500 WP_010344135.1 diguanylate cyclase -
  RAB71_RS14785 (RAB71_14780) - 3504437..3505717 (-) 1281 WP_010344136.1 WD40 repeat domain-containing protein -
  RAB71_RS14790 (RAB71_14785) pilU 3505838..3506968 (-) 1131 WP_010344137.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RAB71_RS14795 (RAB71_14790) pilT 3507096..3508133 (-) 1038 WP_010344138.1 type IV pilus twitching motility protein PilT Machinery gene
  RAB71_RS14800 (RAB71_14795) - 3508256..3508912 (+) 657 WP_040902274.1 YggS family pyridoxal phosphate-dependent enzyme -
  RAB71_RS14805 (RAB71_14800) - 3509230..3510258 (+) 1029 WP_010344140.1 hypothetical protein -
  RAB71_RS14810 (RAB71_14805) proC 3510386..3511231 (+) 846 WP_029562266.1 pyrroline-5-carboxylate reductase -
  RAB71_RS14815 (RAB71_14810) - 3511246..3511698 (+) 453 WP_010344142.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41906.19 Da        Isoelectric Point: 6.8811

>NTDB_id=868295 RAB71_RS14790 WP_010344137.1 3505838..3506968(-) (pilU) [Xanthomonas sacchari strain CFBP4641]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKITPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPSVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRVINFFPEDRRSQLLMDLSLNLRGVVAQQLIPTPDGKGRRVAMEIMLGTPLVQDYIREGEIHKLKDVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRHADSQNEVRLRIKLAQGGDARTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=868295 RAB71_RS14790 WP_010344137.1 3505838..3506968(-) (pilU) [Xanthomonas sacchari strain CFBP4641]
ATGAGCACCATCGACTTCACCTCCTTCCTGAAACTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACCTCGGGCAT
GCCGCCGGCAATCAAGGTGCACGGCAAGATCACGCCGATCACGCAGACGCCGCTGACGCCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACGCCGGCGCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGG
GTCGGGCGCTTCCGCGTCAGTTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACGCGCATTCC
CAGCGTGGACGAGCTGAACCTGCCGCCGGTCATCAAGACTTTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCCACCGGGCACATCATCACC
ATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAGGGCA
TGGACCACGCCATCTCCTTCGCCGAGACCGGCCACCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCGTGATCAACTTCTTCCCCGAAGACCGCCGCAGCCAGTTGCTGATGGATCTGTCGCTGAACCTGCGCGGCGTGGT
CGCGCAGCAGCTGATCCCGACCCCGGACGGCAAGGGCCGCCGCGTGGCCATGGAGATCATGCTCGGCACGCCGCTGGTGC
AGGACTACATCCGCGAGGGCGAGATCCACAAGCTCAAGGACGTGATGAAGGAGTCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGATGCGCTGCGCCACGCCGATTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTGGCCCAGGGCGGCGACGCGCGCACCCTGGCGCAGGGCCTGGACGGGGTCGAGATCGCCG
AGGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.485

97.606

0.649
  pilU Acinetobacter baylyi ADP1

63.585

94.947

0.604
  pilU Vibrio cholerae strain A1552

53.09

94.681

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42

93.085

0.391
  pilT Pseudomonas aeruginosa PAK

41.003

90.16

0.37
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Legionella pneumophila strain ERS1305867

40.597

89.096

0.362
  pilT Legionella pneumophila strain Lp02

40.597

89.096

0.362