Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Q7652_RS02135 Genome accession   NZ_CP131620
Coordinates   411933..413099 (+) Length   388 a.a.
NCBI ID   WP_317852436.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain 2020N08-318     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 406933..418099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7652_RS02110 (Q7652_02100) - 407379..407528 (+) 150 WP_003687747.1 hypothetical protein -
  Q7652_RS02115 (Q7652_02105) - 407752..407859 (+) 108 Protein_407 IS5/IS1182 family transposase -
  Q7652_RS02120 (Q7652_02110) lepB 407964..408983 (-) 1020 WP_003687750.1 signal peptidase I -
  Q7652_RS02125 (Q7652_02115) lepA 409129..410922 (-) 1794 WP_003687751.1 translation elongation factor 4 -
  Q7652_RS02130 (Q7652_02120) - 411064..411765 (-) 702 WP_003687752.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  Q7652_RS02135 (Q7652_02125) pilU 411933..413099 (+) 1167 WP_317852436.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q7652_RS02140 (Q7652_02130) - 413096..414073 (+) 978 WP_003690793.1 DNA polymerase III subunit delta' -
  Q7652_RS02145 (Q7652_02135) - 414077..414427 (+) 351 WP_003687757.1 PilZ domain-containing protein -
  Q7652_RS02150 (Q7652_02140) - 414432..415211 (+) 780 WP_003690796.1 TatD family hydrolase -
  Q7652_RS02155 (Q7652_02145) - 415237..415452 (+) 216 WP_003690799.1 DUF333 domain-containing protein -
  Q7652_RS02160 (Q7652_02150) grxD 415553..415864 (+) 312 WP_003687762.1 Grx4 family monothiol glutaredoxin -
  Q7652_RS02165 (Q7652_02155) upp 415970..416596 (+) 627 WP_003690802.1 uracil phosphoribosyltransferase -
  Q7652_RS02170 (Q7652_02160) - 416619..416939 (+) 321 WP_003690805.1 DUF2322 family protein -
  Q7652_RS02175 (Q7652_02165) - 417108..417749 (+) 642 Protein_419 IS1595 family transposase -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43427.57 Da        Isoelectric Point: 6.1390

>NTDB_id=862831 Q7652_RS02135 WP_317852436.1 411933..413099(+) (pilU) [Neisseria gonorrhoeae strain 2020N08-318]
MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLNLPPALKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKST
TLGMQTFDQHLYQLYEKGEISLQDALKNADSAHDLRLAVQLRSRRAQSSDPDLELSDGGMDFRTGSLT

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=862831 Q7652_RS02135 WP_317852436.1 411933..413099(+) (pilU) [Neisseria gonorrhoeae strain 2020N08-318]
ATGTTTACCGATGAAAATATGACTGCAAAGGAAGAACTGTTCGCATGGCTGCGCCATATGAACAAAAACAAAGGTTCCGA
CCTGTTTGTGACGACCCATTTCCCGCCCGCTATGAAGCTGGACGGCAAAATCACCCGCATCACGGACGAACCGCTGACGG
CGGAAAAATGTATGGAAATCGCCTTTTCGATTATGAGTGCGAAGCAGGCGGAAGAATTTTCATCGACCAACGAGTGCAAC
TTCGCCATCAGCCTGCCGGACACCAGCCGCTTCCGCGTCAATGCGATGATACAGCGCGGTGCGACGGCGTTGGTATTCCG
CGCGATTACCAGCAAGATTCCCAAGTTTGAAAGCCTGAACCTGCCGCCGGCCTTGAAGGATGTTGCGCTGAAAAAACGCG
GGCTGGTTATTTTTGTCGGCGGCACCGGCTCGGGCAAATCGACTTCGCTCGCCTCGCTTATCGACTACCGCAATGAAAAT
TCGTTCGGACACATCATCACCATCGAAGATCCGATCGAGTTTGTCCACGAACACAAAAACTGCATCATTACCCAGCGCGA
GGTCGGCGTGGACACGGAAAACTGGATGGCGGCGTTGAAAAATACGCTGCGTCAGGCGCCGGATGTGATCCTTATCGGCG
AAATCCGCGACCGTGAAACAATGGACTACGCCATCGCCTTTGCCGAAACGGGGCATTTGTGTATGGCGACGCTGCACGCC
AACAGCACCAATCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAGGAGCGGCGCGAACAATTGCTGACGGATTTGTC
GCTCAACCTTCAGGCGTTTATTTCGCAACGCCTCGTTCCGCGAGACGGCGGCAAGGGCAGGGTGGCGGCAGTCGAGGTAC
TGCTCAATTCGCCCCTGATTTCGGAGTTGATTCACAACGGCAACATCCATGAAATCAAAGAAGTGATGAAAAAATCCACT
ACCCTGGGTATGCAGACCTTCGACCAACACCTTTACCAATTGTATGAAAAAGGCGAGATTTCCTTGCAGGATGCCTTGAA
AAATGCCGATTCCGCACATGATTTGCGTTTGGCGGTACAGTTGCGCAGCCGCAGGGCACAAAGTTCCGACCCCGATTTGG
AACTCTCTGATGGCGGTATGGATTTCCGGACGGGCAGTTTGACATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.368

97.938

0.562
  pilU Vibrio cholerae strain A1552

52.66

96.907

0.51
  pilU Acinetobacter baylyi ADP1

57.726

88.402

0.51
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.343

90.979

0.394
  pilT Legionella pneumophila strain Lp02

43.827

83.505

0.366
  pilT Legionella pneumophila strain ERS1305867

43.827

83.505

0.366
  pilT Acinetobacter baylyi ADP1

42.727

85.052

0.363