Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   WCU05_RS27070 Genome accession   NZ_CP147622
Coordinates   5728438..5728863 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain GN06251     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5723438..5733863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WCU05_RS27055 (WCU05_27055) pilX 5724002..5724589 (+) 588 WP_015648054.1 type 4a pilus minor pilin PilX -
  WCU05_RS27060 (WCU05_27060) pilY1 5724601..5728092 (+) 3492 WP_023082062.1 type 4a pilus biogenesis protein PilY1 -
  WCU05_RS27065 (WCU05_27065) pilY2 5728094..5728441 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  WCU05_RS27070 (WCU05_27070) comF 5728438..5728863 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  WCU05_RS27075 (WCU05_27075) ispH 5728910..5729854 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  WCU05_RS27080 (WCU05_27080) fkpB 5729940..5730380 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  WCU05_RS27085 (WCU05_27085) lspA 5730373..5730882 (-) 510 WP_003110420.1 signal peptidase II -
  WCU05_RS27090 (WCU05_27090) ileS 5730875..5733706 (-) 2832 WP_003102617.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=862273 WCU05_RS27070 WP_003094721.1 5728438..5728863(+) (comF) [Pseudomonas aeruginosa strain GN06251]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=862273 WCU05_RS27070 WP_003094721.1 5728438..5728863(+) (comF) [Pseudomonas aeruginosa strain GN06251]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383