Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   WCU20_RS24245 Genome accession   NZ_CP147541
Coordinates   5211115..5211540 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain GN06288     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5206115..5216540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WCU20_RS24230 (WCU20_24230) pilX 5206679..5207266 (+) 588 WP_023090846.1 type 4a pilus minor pilin PilX -
  WCU20_RS24235 (WCU20_24235) pilY1 5207278..5210769 (+) 3492 WP_003123397.1 type 4a pilus biogenesis protein PilY1 -
  WCU20_RS24240 (WCU20_24240) pilY2 5210771..5211118 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  WCU20_RS24245 (WCU20_24245) comF 5211115..5211540 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  WCU20_RS24250 (WCU20_24250) ispH 5211587..5212531 (-) 945 WP_003112824.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  WCU20_RS24255 (WCU20_24255) fkpB 5212617..5213057 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  WCU20_RS24260 (WCU20_24260) lspA 5213050..5213559 (-) 510 WP_003102615.1 signal peptidase II -
  WCU20_RS24265 (WCU20_24265) ileS 5213552..5216383 (-) 2832 WP_003094730.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=860168 WCU20_RS24245 WP_003094721.1 5211115..5211540(+) (comF) [Pseudomonas aeruginosa strain GN06288]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=860168 WCU20_RS24245 WP_003094721.1 5211115..5211540(+) (comF) [Pseudomonas aeruginosa strain GN06288]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383