Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WCU35_RS02170 Genome accession   NZ_CP147532
Coordinates   479041..479508 (-) Length   155 a.a.
NCBI ID   WP_000340159.1    Uniprot ID   -
Organism   Escherichia coli strain GN03139     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 474041..484508
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WCU35_RS02145 (WCU35_02145) rpsG 474182..474652 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  WCU35_RS02150 (WCU35_02150) fusA 474749..476863 (+) 2115 WP_000124700.1 elongation factor G -
  WCU35_RS02155 (WCU35_02155) tuf 476934..478118 (+) 1185 WP_000031783.1 elongation factor Tu -
  WCU35_RS02160 (WCU35_02160) bfd 478301..478495 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  WCU35_RS02165 (WCU35_02165) bfr 478568..479044 (+) 477 WP_000675515.1 bacterioferritin -
  WCU35_RS02170 (WCU35_02170) pilD 479041..479508 (-) 468 WP_000340159.1 A24 family peptidase Machinery gene
  WCU35_RS02175 (WCU35_02175) rpsJ 479887..480198 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  WCU35_RS02180 (WCU35_02180) rplC 480231..480860 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  WCU35_RS02185 (WCU35_02185) rplD 480871..481476 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  WCU35_RS02190 (WCU35_02190) rplW 481473..481775 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  WCU35_RS02195 (WCU35_02195) rplB 481793..482614 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  WCU35_RS02200 (WCU35_02200) rpsS 482631..482909 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  WCU35_RS02205 (WCU35_02205) rplV 482924..483256 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  WCU35_RS02210 (WCU35_02210) rpsC 483274..483975 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  WCU35_RS02215 (WCU35_02215) rplP 483988..484398 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17034.17 Da        Isoelectric Point: 8.1255

>NTDB_id=859934 WCU35_RS02170 WP_000340159.1 479041..479508(-) (pilD) [Escherichia coli strain GN03139]
MDATLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=859934 WCU35_RS02170 WP_000340159.1 479041..479508(-) (pilD) [Escherichia coli strain GN03139]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCAGCCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACATGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGCTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(11-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.882

81.935

0.368