Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   V8955_RS13525 Genome accession   NZ_CP146564
Coordinates   2631924..2632688 (+) Length   254 a.a.
NCBI ID   WP_001136250.1    Uniprot ID   -
Organism   Escherichia coli strain B1.H1.18     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 2626924..2637688
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8955_RS13505 (V8955_13505) acpT 2627878..2628465 (+) 588 WP_000285765.1 4'-phosphopantetheinyl transferase AcpT -
  V8955_RS13510 (V8955_13510) nikA 2628576..2630150 (+) 1575 WP_000493127.1 nickel ABC transporter substrate-binding protein -
  V8955_RS13515 (V8955_13515) nikB 2630150..2631094 (+) 945 WP_000947088.1 nickel ABC transporter permease subunit NikB -
  V8955_RS13520 (V8955_13520) nikC 2631091..2631924 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  V8955_RS13525 (V8955_13525) amiE 2631924..2632688 (+) 765 WP_001136250.1 nickel import ATP-binding protein NikD Regulator
  V8955_RS13530 (V8955_13530) nikE 2632685..2633491 (+) 807 WP_000173693.1 nickel import ATP-binding protein NikE -
  V8955_RS13535 (V8955_13535) nikR 2633496..2633897 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  V8955_RS13540 (V8955_13540) - 2634096..2634842 (+) 747 WP_001322918.1 GntR family transcriptional regulator -
  V8955_RS13545 (V8955_13545) - 2634867..2635340 (+) 474 WP_001377099.1 PTS sugar transporter subunit IIA -
  V8955_RS13550 (V8955_13550) - 2635337..2635618 (+) 282 WP_000084021.1 PTS sugar transporter subunit IIB -
  V8955_RS13555 (V8955_13555) - 2635695..2637053 (+) 1359 WP_001306345.1 PTS galactitol transporter subunit IIC -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.40 Da        Isoelectric Point: 6.6882

>NTDB_id=855552 V8955_RS13525 WP_001136250.1 2631924..2632688(+) (amiE) [Escherichia coli strain B1.H1.18]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGSSGSGKSLTCAAALGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAALEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=855552 V8955_RS13525 WP_001136250.1 2631924..2632688(+) (amiE) [Escherichia coli strain B1.H1.18]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAACCGCTGGTGCACGGCGTATCGTTAACTCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCAGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCCGCACTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTTCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCCACGCTTACCGCTGCTTTAGAAGCGGTGGGGTTGGAAAACGCCGCGCGCGTGT
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGTTGTGTGAATCGCCG
TTTATCATCGCCGATGAACCGACCACCGATCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACACGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAATGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398