Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   V8957_RS04540 Genome accession   NZ_CP146556
Coordinates   994286..995050 (-) Length   254 a.a.
NCBI ID   WP_001136198.1    Uniprot ID   -
Organism   Escherichia coli strain B1.H1.39     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 989286..1000050
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8957_RS04530 (V8957_04530) nikR 993077..993478 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  V8957_RS04535 (V8957_04535) nikE 993483..994289 (-) 807 WP_001521512.1 nickel import ATP-binding protein NikE -
  V8957_RS04540 (V8957_04540) amiE 994286..995050 (-) 765 WP_001136198.1 nickel import ATP-binding protein NikD Regulator
  V8957_RS04545 (V8957_04545) nikC 995050..995883 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  V8957_RS04550 (V8957_04550) nikB 995880..996824 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  V8957_RS04555 (V8957_04555) nikA 996824..998398 (-) 1575 WP_000953368.1 nickel ABC transporter substrate-binding protein -
  V8957_RS04560 (V8957_04560) acpT 998509..999096 (-) 588 WP_000285781.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26843.46 Da        Isoelectric Point: 6.6886

>NTDB_id=855451 V8957_RS04540 WP_001136198.1 994286..995050(-) (amiE) [Escherichia coli strain B1.H1.39]
MPQQIELRDIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=855451 V8957_RS04540 WP_001136198.1 994286..995050(-) (amiE) [Escherichia coli strain B1.H1.39]
ATGCCGCAACAGATTGAACTGCGCGATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTTAA
ACGCGGGCGTGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCACTGACCTGCGCCGCGACGCTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGTGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTACACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGTTGGAAAACGCCGCGCGCGTGT
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGTTGTGTGAATCGCCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACACGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

42

98.425

0.413
  amiE Streptococcus thermophilus LMD-9

42

98.425

0.413
  amiE Streptococcus salivarius strain HSISS4

40.8

98.425

0.402