Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   V6M96_RS01355 Genome accession   NZ_CP146250
Coordinates   299431..300195 (-) Length   254 a.a.
NCBI ID   WP_047644476.1    Uniprot ID   -
Organism   Escherichia coli strain L26     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 294431..305195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6M96_RS01330 (V6M96_01330) yhhJ 296273..297397 (+) 1125 WP_001314210.1 ABC transporter permease -
  V6M96_RS01335 (V6M96_01335) - 297470..297745 (+) 276 WP_072686259.1 type II toxin-antitoxin system HicA family toxin -
  V6M96_RS01340 (V6M96_01340) - 297742..298101 (+) 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin -
  V6M96_RS01345 (V6M96_01345) nikR 298221..298622 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  V6M96_RS01350 (V6M96_01350) nikE 298628..299434 (-) 807 WP_032288306.1 nickel import ATP-binding protein NikE -
  V6M96_RS01355 (V6M96_01355) amiE 299431..300195 (-) 765 WP_047644476.1 nickel import ATP-binding protein NikD Regulator
  V6M96_RS01360 (V6M96_01360) nikC 300195..301028 (-) 834 WP_072686260.1 nickel ABC transporter permease subunit NikC -
  V6M96_RS01365 (V6M96_01365) nikB 301025..301969 (-) 945 WP_000947082.1 nickel ABC transporter permease subunit NikB -
  V6M96_RS01370 (V6M96_01370) nikA 301969..303543 (-) 1575 WP_000953368.1 nickel ABC transporter substrate-binding protein -
  V6M96_RS01375 (V6M96_01375) acpT 303654..304241 (-) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26861.52 Da        Isoelectric Point: 7.0949

>NTDB_id=855032 V6M96_RS01355 WP_047644476.1 299431..300195(-) (amiE) [Escherichia coli strain L26]
MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLRTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=855032 V6M96_RS01355 WP_047644476.1 299431..300195(-) (amiE) [Escherichia coli strain L26]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGAA
ACGCGGGCGTGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTCTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTACGCACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCACGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTTTGCGAATCACCG
TTTATCATCGCCGATGAACCAACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAGGCACCGGGGATGCTGCTGGTCACCCATGATATGGGCGTAGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCGCACGGTAAGATTGTCGAACAGGGCGATGTCGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398