Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   V6591_RS01300 Genome accession   NZ_CP146019
Coordinates   286428..287192 (-) Length   254 a.a.
NCBI ID   WP_032218476.1    Uniprot ID   -
Organism   Escherichia coli strain ECO8855     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 281428..292192
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6591_RS01275 (V6591_01275) yhhJ 283284..284408 (+) 1125 WP_001314210.1 ABC transporter permease -
  V6591_RS01280 (V6591_01280) - 284512..284742 (+) 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin -
  V6591_RS01285 (V6591_01285) - 284739..285098 (+) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  V6591_RS01290 (V6591_01290) nikR 285218..285619 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  V6591_RS01295 (V6591_01295) nikE 285625..286431 (-) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  V6591_RS01300 (V6591_01300) amiE 286428..287192 (-) 765 WP_032218476.1 nickel import ATP-binding protein NikD Regulator
  V6591_RS01305 (V6591_01305) nikC 287192..288025 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  V6591_RS01310 (V6591_01310) nikB 288022..288966 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  V6591_RS01315 (V6591_01315) nikA 288966..290540 (-) 1575 WP_000953353.1 nickel ABC transporter substrate-binding protein -
  V6591_RS01320 (V6591_01320) acpT 290651..291238 (-) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26863.44 Da        Isoelectric Point: 6.5992

>NTDB_id=854470 V6591_RS01300 WP_032218476.1 286428..287192(-) (amiE) [Escherichia coli strain ECO8855]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCTATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=854470 V6591_RS01300 WP_032218476.1 286428..287192(-) (amiE) [Escherichia coli strain ECO8855]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCACCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398