Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   V6S32_RS21255 Genome accession   NZ_CP145683
Coordinates   4311018..4311695 (+) Length   225 a.a.
NCBI ID   WP_000178175.1    Uniprot ID   A0A0H2Z3H2
Organism   Escherichia coli strain S1-KEN-07-A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4306018..4316695
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6S32_RS21220 (V6S32_21225) gspG 4306498..4306935 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  V6S32_RS21225 (V6S32_21230) gspH 4306943..4307452 (+) 510 WP_001539909.1 type II secretion system minor pseudopilin GspH -
  V6S32_RS21230 (V6S32_21235) gspI 4307449..4307826 (+) 378 WP_001041748.1 type II secretion system minor pseudopilin GspI -
  V6S32_RS21235 (V6S32_21240) gspJ 4307819..4308406 (+) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  V6S32_RS21240 (V6S32_21245) gspK 4308399..4309382 (+) 984 WP_001058513.1 type II secretion system minor pseudopilin GspK -
  V6S32_RS21245 (V6S32_21250) gspL 4309397..4310560 (+) 1164 Protein_4147 type II secretion system protein GspL -
  V6S32_RS21250 (V6S32_21255) gspM 4310557..4311018 (+) 462 WP_001298207.1 type II secretion system protein GspM -
  V6S32_RS21255 (V6S32_21260) pilD 4311018..4311695 (+) 678 WP_000178175.1 prepilin peptidase GspO Machinery gene
  V6S32_RS21260 (V6S32_21265) bfr 4311724..4312200 (-) 477 WP_000675504.1 bacterioferritin -
  V6S32_RS21265 (V6S32_21270) bfd 4312272..4312466 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  V6S32_RS21270 (V6S32_21275) chiA 4312635..4315337 (-) 2703 WP_000773146.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24870.75 Da        Isoelectric Point: 7.6364

>NTDB_id=852216 V6S32_RS21255 WP_000178175.1 4311018..4311695(+) (pilD) [Escherichia coli strain S1-KEN-07-A]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=852216 V6S32_RS21255 WP_000178175.1 4311018..4311695(+) (pilD) [Escherichia coli strain S1-KEN-07-A]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z3H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387