Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   V6S33_RS22120 Genome accession   NZ_CP145679
Coordinates   4504707..4505471 (+) Length   254 a.a.
NCBI ID   WP_001136232.1    Uniprot ID   Q0TBX9
Organism   Escherichia coli strain S1-NGR-02-A     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4499707..4510471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6S33_RS22100 (V6S33_22100) acpT 4500661..4501248 (+) 588 WP_000285774.1 4'-phosphopantetheinyl transferase AcpT -
  V6S33_RS22105 (V6S33_22105) nikA 4501359..4502933 (+) 1575 WP_000953362.1 nickel ABC transporter substrate-binding protein -
  V6S33_RS22110 (V6S33_22110) nikB 4502933..4503877 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  V6S33_RS22115 (V6S33_22115) nikC 4503874..4504707 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  V6S33_RS22120 (V6S33_22120) amiE 4504707..4505471 (+) 765 WP_001136232.1 nickel import ATP-binding protein NikD Regulator
  V6S33_RS22125 (V6S33_22125) nikE 4505468..4506274 (+) 807 WP_000173707.1 nickel import ATP-binding protein NikE -
  V6S33_RS22130 (V6S33_22130) nikR 4506280..4506681 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  V6S33_RS22135 (V6S33_22135) - 4506801..4507160 (-) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  V6S33_RS22140 (V6S33_22140) - 4507157..4507432 (-) 276 Protein_4327 type II toxin-antitoxin system HicA family toxin -
  V6S33_RS22145 (V6S33_22145) yhhJ 4507505..4508629 (-) 1125 WP_001216257.1 ABC transporter permease -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.40 Da        Isoelectric Point: 6.6882

>NTDB_id=852133 V6S33_RS22120 WP_001136232.1 4504707..4505471(+) (amiE) [Escherichia coli strain S1-NGR-02-A]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=852133 V6S33_RS22120 WP_001136232.1 4504707..4505471(+) (amiE) [Escherichia coli strain S1-NGR-02-A]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAGGCACCGGGGATGCTGCTGGTCACCCATGATATGGGCGTAGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCGCACGGTAAGATTGTCGAACAGGGCGATGTCGAAACGCTGTTTAACGCCCCCAAACATGCGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0TBX9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398