Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   QUF33_RS18365 Genome accession   NZ_AP027246
Coordinates   3691588..3692055 (-) Length   155 a.a.
NCBI ID   WP_000340167.1    Uniprot ID   A7ZSL1
Organism   Escherichia coli strain JNE120393     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3686588..3697055
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUF33_RS18340 (JNE120393_35020) rpsG 3686730..3687200 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  QUF33_RS18345 (JNE120393_35030) fusA 3687297..3689411 (+) 2115 WP_000124700.1 elongation factor G -
  QUF33_RS18350 (JNE120393_35040) tuf 3689482..3690666 (+) 1185 WP_000031783.1 elongation factor Tu -
  QUF33_RS18355 bfd 3690849..3691042 (+) 194 Protein_3585 bacterioferritin-associated ferredoxin -
  QUF33_RS18360 (JNE120393_35050) bfr 3691115..3691591 (+) 477 WP_000675515.1 bacterioferritin -
  QUF33_RS18365 (JNE120393_35060) pilD 3691588..3692055 (-) 468 WP_000340167.1 A24 family peptidase Machinery gene
  QUF33_RS18370 (JNE120393_35070) rpsJ 3692434..3692745 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  QUF33_RS18375 (JNE120393_35080) rplC 3692778..3693407 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  QUF33_RS18380 (JNE120393_35090) rplD 3693418..3694023 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  QUF33_RS18385 (JNE120393_35100) rplW 3694020..3694322 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  QUF33_RS18390 (JNE120393_35110) rplB 3694340..3695161 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  QUF33_RS18395 (JNE120393_35120) rpsS 3695178..3695456 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  QUF33_RS18400 (JNE120393_35130) rplV 3695471..3695803 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  QUF33_RS18405 (JNE120393_35140) rpsC 3695821..3696522 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  QUF33_RS18410 (JNE120393_35150) rplP 3696535..3696945 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16984.17 Da        Isoelectric Point: 8.0293

>NTDB_id=85108 QUF33_RS18365 WP_000340167.1 3691588..3692055(-) (pilD) [Escherichia coli strain JNE120393]
MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDCLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=85108 QUF33_RS18365 WP_000340167.1 3691588..3692055(-) (pilD) [Escherichia coli strain JNE120393]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACTGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZSL1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361

Multiple sequence alignment