Detailed information NTG0851020

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: V6S48_RS14755
• Genome accession: NZ_CP145620
• Coordinates: 3016960..3017676 (-)
• Length: 238 a.a.
• NCBI ID: WP_238086664.1
• Uniprot ID: -
• Organism: Escherichia coli strain S1-ESP-03-A
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3011960..3022676

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V6S48_RS14730 (V6S48_14730)	-	3013083..3013537 (+)	455	Protein_2890	IS110 family transposase	-
V6S48_RS14735 (V6S48_14735)	-	3013963..3014513 (+)	551	Protein_2891	transposase	-
V6S48_RS14740 (V6S48_14740)	-	3014588..3015764 (+)	1177	Protein_2892	IS3 family transposase	-
V6S48_RS14745 (V6S48_14745)	-	3015940..3016176 (-)	237	Protein_2893	transposase	-
V6S48_RS14750 (V6S48_14750)	eptO	3016469..3016867 (-)	399	WP_115725091.1	GspS family T2SS pilot lipoprotein variant EptO	-
V6S48_RS14755 (V6S48_14755)	pilD	3016960..3017676 (-)	717	WP_238086664.1	A24 family peptidase	Machinery gene
V6S48_RS14760 (V6S48_14760)	-	3017851..3018363 (-)	513	WP_115725081.1	type II secretion system protein M	-
V6S48_RS14765 (V6S48_14765)	gspL	3018350..3019627 (-)	1278	WP_238086661.1	type II secretion system protein GspL	-
V6S48_RS14770 (V6S48_14770)	gspK	3019561..3020538 (-)	978	WP_115725082.1	type II secretion system minor pseudopilin GspK	-
V6S48_RS14775 (V6S48_14775)	gspJ	3020535..3021134 (-)	600	WP_115725083.1	type II secretion system minor pseudopilin GspJ	-
V6S48_RS14780 (V6S48_14780)	gspI	3021131..3021478 (-)	348	WP_115725084.1	type II secretion system minor pseudopilin GspI	-
V6S48_RS14785 (V6S48_14785)	gspH	3021493..3022047 (-)	555	WP_115725085.1	type II secretion system minor pseudopilin GspH	-
V6S48_RS14790 (V6S48_14790)	gspG	3022044..3022478 (-)	435	WP_115725086.1	type II secretion system major pseudopilin GspG	-

Sequence

Protein

• Length: 238 a.a.
• Molecular weight: 26375.64 Da
• Isoelectric Point: 8.1587

>NTDB_id=851020 V6S48_RS14755 WP_238086664.1 3016960..3017676(-) (pilD) [Escherichia coli strain S1-ESP-03-A]
MLERRWRREALMLLAQNVPESGPAFNLMIPQSHCPSCFHPLGIRDNIPLLSYLFLKGKSRCCGERIPVYYPLVEITNSVL
FILAASRFPPGLTLAAAWLFMSMLLVLAVIDCHTALLPDVLTLPLLWLGLLFSLQRGVVPLEEAVVGAVSGYLCLWGLYW
LFRFATGREGLGYGDFKLAAALGAWVGWQVLPSVLLFASVSGLIIMVLLRIVTSTDFTRPLPFGPWLALSGVCHFLLM

Nucleotide

• Length: 717 bp

>NTDB_id=851020 V6S48_RS14755 WP_238086664.1 3016960..3017676(-) (pilD) [Escherichia coli strain S1-ESP-03-A]
ATGTTGGAGAGGCGGTGGCGGCGTGAAGCGCTGATGCTATTGGCACAGAATGTGCCTGAGTCCGGGCCGGCCTTTAACCT
GATGATACCGCAGTCTCATTGTCCCAGCTGTTTTCATCCGCTCGGCATCAGGGATAATATTCCGCTGTTAAGCTACCTAT
TTCTGAAGGGGAAATCACGCTGTTGTGGCGAGCGTATTCCCGTTTACTACCCGCTAGTCGAAATAACGAACTCAGTGTTA
TTCATTCTGGCCGCGAGCAGGTTTCCACCAGGGCTGACTTTAGCGGCTGCCTGGCTTTTTATGTCCATGCTGCTGGTTCT
GGCGGTTATTGACTGCCACACGGCGCTGCTGCCAGATGTGCTGACGTTGCCCTTGCTCTGGCTTGGCCTGTTATTCAGTT
TACAGAGAGGGGTTGTCCCCTTAGAGGAGGCGGTAGTGGGTGCGGTGTCTGGGTATCTCTGTCTGTGGGGACTGTACTGG
CTTTTTCGTTTCGCTACCGGGAGGGAAGGGTTAGGTTACGGTGATTTTAAGCTAGCTGCGGCATTGGGAGCATGGGTAGG
TTGGCAGGTATTGCCCTCTGTGCTTCTGTTTGCCTCAGTTAGCGGGTTAATAATAATGGTGCTGTTGCGTATTGTGACAT
CTACGGACTTTACCCGTCCGCTGCCTTTTGGGCCGTGGCTCGCTTTGAGCGGAGTGTGCCATTTTTTGTTGATGTAG

Domains

Predicted by InterProScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-88)

• Type IV leader peptidase family (Peptidase_A24) (99-206)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	45.378	100	0.454
pilD	Vibrio campbellii strain DS40M4	41.597	100	0.416
pilD	Neisseria gonorrhoeae MS11	42.308	98.319	0.416
pilD	Acinetobacter nosocomialis M2	38.397	99.58	0.382
pilD	Acinetobacter baumannii D1279779	37.975	99.58	0.378