Detailed information NTG0849566

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: QU514_RS22360
• Genome accession: NZ_CP128794
• Coordinates: 4507183..4507983 (+)
• Length: 266 a.a.
• NCBI ID: WP_023322076.1
• Uniprot ID: -
• Organism: Klebsiella variicola strain KP599
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4502183..4512983

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QU514_RS22325 (QU514_22295)	gspH	4502336..4502851 (+)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-
QU514_RS22330 (QU514_22300)	gspI	4502848..4503213 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
QU514_RS22335 (QU514_22305)	gspJ	4503210..4503806 (+)	597	WP_008807379.1	type II secretion system minor pseudopilin GspJ	-
QU514_RS22340 (QU514_22310)	gspK	4503803..4504783 (+)	981	WP_023322079.1	type II secretion system minor pseudopilin GspK	-
QU514_RS22345 (QU514_22315)	gspL	4504776..4505972 (+)	1197	WP_032738815.1	type II secretion system protein GspL	-
QU514_RS22350 (QU514_22320)	-	4505965..4506450 (+)	486	WP_012968921.1	type II secretion system protein M	-
QU514_RS22355 (QU514_22325)	-	4506440..4507186 (+)	747	WP_048267462.1	type II secretion system protein N	-
QU514_RS22360 (QU514_22330)	pilD	4507183..4507983 (+)	801	WP_023322076.1	prepilin peptidase	Machinery gene
QU514_RS22365 (QU514_22335)	hrpB	4508030..4510459 (-)	2430	WP_109909965.1	ATP-dependent helicase HrpB	-
QU514_RS22370 (QU514_22340)	thpR	4510532..4511068 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
QU514_RS22375 (QU514_22345)	sfsA	4511068..4511784 (+)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
QU514_RS22380 (QU514_22350)	dksA	4511947..4512402 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29445.19 Da
• Isoelectric Point: 8.7151

>NTDB_id=849566 QU514_RS22360 WP_023322076.1 4507183..4507983(+) (pilD) [Klebsiella variicola strain KP599]
MTILTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=849566 QU514_RS22360 WP_023322076.1 4507183..4507983(+) (pilD) [Klebsiella variicola strain KP599]
ATGACGATATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCATCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425