Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   V5A90_RS02720 Genome accession   NZ_CP144885
Coordinates   568252..569685 (-) Length   477 a.a.
NCBI ID   WP_195808897.1    Uniprot ID   -
Organism   Escherichia coli strain TC_EC10     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 563252..574685
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5A90_RS02685 (V5A90_02690) elbB 563944..564597 (+) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -
  V5A90_RS02690 (V5A90_02695) mtgA 564594..565322 (+) 729 WP_000047073.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  V5A90_RS02695 (V5A90_02700) yrbL 565319..565951 (-) 633 WP_000620398.1 PhoP regulatory network protein YrbL -
  V5A90_RS02700 (V5A90_02705) npr 566164..566436 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  V5A90_RS02705 (V5A90_02710) rapZ 566433..567287 (-) 855 WP_000243741.1 RNase adapter RapZ -
  V5A90_RS02710 (V5A90_02715) ptsN 567333..567824 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  V5A90_RS02715 (V5A90_02720) hpf 567942..568229 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  V5A90_RS02720 (V5A90_02725) rpoN/rpoN1 568252..569685 (-) 1434 WP_195808897.1 RNA polymerase factor sigma-54 Machinery gene
  V5A90_RS02725 (V5A90_02730) lptB 569733..570458 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  V5A90_RS02730 (V5A90_02735) lptA 570465..571022 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  V5A90_RS02735 (V5A90_02740) lptC 570991..571566 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  V5A90_RS02740 (V5A90_02745) kdsC 571563..572129 (-) 567 WP_000030006.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  V5A90_RS02745 (V5A90_02750) kdsD 572150..573136 (-) 987 WP_001309787.1 arabinose-5-phosphate isomerase KdsD -
  V5A90_RS02750 (V5A90_02755) yrbG 573150..574127 (-) 978 WP_000922881.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53992.85 Da        Isoelectric Point: 4.4085

>NTDB_id=847768 V5A90_RS02720 WP_195808897.1 568252..569685(-) (rpoN/rpoN1) [Escherichia coli strain TC_EC10]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHKEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=847768 V5A90_RS02720 WP_195808897.1 568252..569685(-) (rpoN/rpoN1) [Escherichia coli strain TC_EC10]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATA
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGATACTGACCGCGCCATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATTATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACGGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCCTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465