Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   V2191_RS01460 Genome accession   NZ_CP143907
Coordinates   317798..318223 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain PA7_SE3_14.22_ST654_NDM1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 312798..323223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V2191_RS01445 (V2191_01445) pilX 313362..313949 (+) 588 WP_034037187.1 type 4a pilus minor pilin PilX -
  V2191_RS01450 (V2191_01450) pilY1 313961..317452 (+) 3492 WP_003123397.1 type 4a pilus biogenesis protein PilY1 -
  V2191_RS01455 (V2191_01455) pilY2 317454..317801 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  V2191_RS01460 (V2191_01460) comF 317798..318223 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  V2191_RS01465 (V2191_01465) ispH 318270..319214 (-) 945 WP_003112824.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  V2191_RS01470 (V2191_01470) fkpB 319300..319740 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  V2191_RS01475 (V2191_01475) lspA 319733..320242 (-) 510 WP_003102615.1 signal peptidase II -
  V2191_RS01480 (V2191_01480) ileS 320235..323066 (-) 2832 WP_003102617.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=845341 V2191_RS01460 WP_003094721.1 317798..318223(+) (comF) [Pseudomonas aeruginosa strain PA7_SE3_14.22_ST654_NDM1]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=845341 V2191_RS01460 WP_003094721.1 317798..318223(+) (comF) [Pseudomonas aeruginosa strain PA7_SE3_14.22_ST654_NDM1]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383