Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   V2192_RS03125 Genome accession   NZ_CP143906
Coordinates   679063..680847 (+) Length   594 a.a.
NCBI ID   WP_003096276.1    Uniprot ID   A0A0H2ZIS8
Organism   Pseudomonas aeruginosa strain PA15_L5_37.22_ST773_VIM2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 674063..685847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V2192_RS03105 (V2192_03105) argE 674301..675455 (-) 1155 WP_033937909.1 acetylornithine deacetylase -
  V2192_RS03110 (V2192_03110) - 675555..676823 (-) 1269 WP_003100079.1 inorganic phosphate transporter -
  V2192_RS03115 (V2192_03115) - 676870..677547 (-) 678 WP_003096272.1 TIGR00153 family protein -
  V2192_RS03120 (V2192_03120) - 677661..679025 (+) 1365 WP_009314472.1 inorganic triphosphatase -
  V2192_RS03125 (V2192_03125) pilB 679063..680847 (+) 1785 WP_003096276.1 GspE/PulE family protein Machinery gene
  V2192_RS03130 (V2192_03130) - 681069..681521 (+) 453 WP_009314473.1 NfeD family protein -
  V2192_RS03135 (V2192_03135) - 681653..681982 (+) 330 WP_003096280.1 DUF2388 domain-containing protein -
  V2192_RS03140 (V2192_03140) gcvP 682017..684893 (-) 2877 WP_031285798.1 aminomethyl-transferring glycine dehydrogenase -
  V2192_RS03145 (V2192_03145) gcvH 685067..685456 (-) 390 WP_003107397.1 glycine cleavage system protein GcvH -

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 65981.61 Da        Isoelectric Point: 6.5096

>NTDB_id=845239 V2192_RS03125 WP_003096276.1 679063..680847(+) (pilB) [Pseudomonas aeruginosa strain PA15_L5_37.22_ST773_VIM2]
MSVFATAAQDRLLDLGDLLRELVAQGRVGQEQAEQCLAIRRSAVRNQQHPLEFLAAQQVEDLKRPGRKLDLETLTQWLAE
YAGQPYLRIDPLKIDVAAVTPLMSYAFAQRHGILAVALSPEEVTIASAQPFVQGWESNLVHVLKRPIKRVVANPADIQRY
TVEFFRLARSVSGATSPDQKISGVGNFEQLLNLGAADQEPDANDAHIVTIVDWLFQYAFQQRASDIHIEPRREQGTVRFR
IDGVLHNVYQFPPQVTMAVVSRLKSLGRMNVAEKRKPQDGRVKTKTPDGAEVELRLSTLPTAFGEKMVMRIFDPEVLLKS
FDQLGFSADDLRRWQSMTSQPNGIILVTGPTGSGKTTTLYTTLKQLATDEVNVCTIEDPIEMIEPSFNQMQVQHNIDLTF
ASGVRALLRQDPDIIMVGEIRDLETAEMAIQAALTGHLVLSTLHTNDSPTAITRLLELGVPYYLIRATVLGVMAQRLVRT
LCPHCKAPMQLADSDWHELTRPWSAPPPSGAHQAVGCLECRDTGYRGRAGVYEIMLLSDSLKELITADTDLVALRRQAYK
EGTRSLRLSGAQKVAAGISTLEEVLRVTPQSEQK

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=845239 V2192_RS03125 WP_003096276.1 679063..680847(+) (pilB) [Pseudomonas aeruginosa strain PA15_L5_37.22_ST773_VIM2]
ATGTCCGTGTTCGCCACCGCCGCCCAGGACCGCCTGCTGGACCTCGGCGACCTGCTCCGCGAGCTGGTCGCCCAGGGCCG
GGTCGGGCAGGAGCAGGCCGAACAGTGCCTGGCGATCCGCCGCAGCGCGGTGCGCAACCAGCAGCATCCGCTGGAGTTCC
TCGCCGCGCAGCAGGTCGAGGATCTCAAGCGCCCCGGACGCAAGCTCGACCTGGAGACCCTCACCCAGTGGCTGGCCGAG
TACGCCGGCCAGCCCTACCTGCGCATCGACCCGCTGAAGATCGACGTCGCCGCGGTGACTCCGCTGATGTCCTACGCCTT
CGCCCAGCGCCATGGCATCCTCGCCGTCGCGCTGAGTCCGGAGGAAGTCACCATCGCCAGCGCCCAGCCCTTCGTACAGG
GCTGGGAAAGCAACCTGGTGCATGTGCTCAAGCGACCGATCAAGCGGGTGGTGGCGAACCCGGCGGACATCCAGCGCTAC
ACCGTCGAGTTCTTCCGCCTGGCCCGCTCGGTCAGCGGCGCCACCTCGCCGGACCAGAAGATCAGCGGCGTCGGCAACTT
CGAGCAGTTGCTCAACCTCGGCGCCGCCGACCAGGAGCCGGACGCCAACGACGCGCACATCGTCACCATCGTCGACTGGC
TGTTCCAGTACGCCTTCCAGCAGCGCGCCAGCGACATCCACATCGAGCCGCGCCGCGAGCAGGGCACCGTGCGCTTCCGT
ATCGACGGCGTGCTGCACAACGTCTACCAGTTCCCGCCGCAGGTGACCATGGCGGTGGTCAGCCGCCTGAAGTCGCTGGG
ACGGATGAACGTGGCGGAGAAACGCAAGCCGCAGGACGGCCGGGTCAAGACCAAGACCCCGGACGGCGCCGAGGTGGAGC
TGCGCCTGTCGACCCTGCCCACCGCCTTCGGCGAGAAGATGGTGATGCGCATCTTCGACCCCGAGGTGCTGCTCAAGAGC
TTCGACCAGCTCGGCTTCTCCGCCGACGACCTGCGCCGCTGGCAGAGCATGACCAGCCAGCCCAACGGCATCATCCTGGT
CACCGGCCCCACCGGCTCGGGCAAGACCACCACGCTCTACACCACGCTCAAGCAACTGGCCACTGACGAGGTGAACGTCT
GCACCATCGAGGACCCGATCGAGATGATCGAGCCCTCGTTCAACCAGATGCAGGTCCAGCACAACATCGACCTGACCTTC
GCCAGCGGCGTACGCGCCCTGCTGCGGCAGGACCCGGACATCATCATGGTCGGCGAGATCCGCGACCTGGAGACCGCCGA
GATGGCGATCCAGGCGGCGCTCACCGGGCACCTGGTGCTCTCCACCCTGCACACCAACGACTCGCCCACGGCGATCACCC
GCCTGCTCGAACTGGGCGTGCCCTACTACCTGATCCGCGCCACGGTGCTCGGGGTCATGGCCCAGCGCCTGGTGCGCACA
CTCTGCCCGCACTGCAAGGCGCCGATGCAACTGGCGGACAGCGACTGGCACGAACTGACCCGCCCCTGGAGCGCCCCGCC
GCCCAGCGGCGCGCACCAGGCCGTGGGTTGCCTGGAGTGCCGCGACACCGGCTATCGCGGGCGCGCCGGCGTCTATGAAA
TCATGCTGCTCAGCGATAGCCTGAAAGAACTGATCACCGCCGACACCGACCTGGTCGCCCTGCGCCGCCAGGCGTACAAG
GAAGGCACCCGCAGCCTGCGCCTGTCCGGCGCGCAGAAGGTCGCCGCAGGTATCAGTACGCTGGAGGAAGTACTGCGGGT
GACCCCGCAGAGCGAACAGAAGTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(216-480)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(69-167)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZIS8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.682

90.067

0.375