Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   QRD24_RS23315 Genome accession   NZ_CP127874
Coordinates   4453638..4455980 (-) Length   780 a.a.
NCBI ID   WP_286127763.1    Uniprot ID   -
Organism   Priestia megaterium strain ELP1.24a     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4448638..4460980
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QRD24_RS23290 (QRD24_23285) - 4449031..4449207 (-) 177 WP_013059305.1 hypothetical protein -
  QRD24_RS23295 (QRD24_23290) - 4449374..4450258 (+) 885 WP_231504553.1 N-acetylmuramoyl-L-alanine amidase -
  QRD24_RS23300 (QRD24_23295) dtd 4450249..4450710 (-) 462 WP_028410584.1 D-aminoacyl-tRNA deacylase -
  QRD24_RS23305 (QRD24_23300) - 4450726..4452924 (-) 2199 WP_013059308.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  QRD24_RS23310 (QRD24_23305) - 4453110..4453622 (-) 513 WP_013059309.1 adenine phosphoribosyltransferase -
  QRD24_RS23315 (QRD24_23310) recJ 4453638..4455980 (-) 2343 WP_286127763.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  QRD24_RS23320 (QRD24_23315) secDF 4456121..4458358 (-) 2238 WP_050688551.1 protein translocase subunit SecDF -
  QRD24_RS23325 (QRD24_23320) - 4458496..4458789 (-) 294 WP_025750585.1 post-transcriptional regulator -
  QRD24_RS23330 (QRD24_23325) spoVB 4458914..4460464 (+) 1551 WP_060745487.1 stage V sporulation protein B -

Sequence

Protein


Download         Length: 780 a.a.        Molecular weight: 87807.43 Da        Isoelectric Point: 4.7708

>NTDB_id=845037 QRD24_RS23315 WP_286127763.1 4453638..4455980(-) (recJ) [Priestia megaterium strain ELP1.24a]
MLQSKKRWNVQECDEQLVNEFVENLNITPLVASLLLNRGMTTVEAAREFLHVDAQTFHDPFLLHDMDKAVARIQQAIDHN
EKIMIYGDYDADGVSSTSVMLSALQQLGADVDFYIPNRFTEGYGPNKAAFDKISAGGYQLVITVDTGISAVEETAYAREL
NFDLIITDHHEPGPVLPDAYAIIHPKLPGSTYPFKELAGVGVAFKLAHALLGELPEHLLELAVIGTIADLVPLVDENRLI
ARKGLDYLKLTKRTGIEALLSVCGVKREEINEDTIGFAMAPRINAVGRLQGADPAVHLLMTTNAEEAKGLAKEIDALNKE
RQQIVNDITEEAVEFVESMYPPDENSVLIVEGEGWNAGVVGIVASRLVEKFYRPTIVLSIDSENRIAKGSARSIEGFDLF
ANLSKCRDILPHFGGHPMAAGMTLNSDDVEELRRRLNEQASNVLTEQDFIPITSVDTVCTTEEITLENIEQMSALAPFGM
HNPKPKVLIKDVHTTSMRKIGANKNHLKLVFEDSGVSVDAVGFGLGYILDEVSPIAKVSLVGELSINEWNNMRKPQLMVE
DISVDEWQMFDYRSAVDVKRFFQQVFTDQMKVVAFHASTIDKYKSLLPMEHVVLIQDEEQAKACMFAGEQIVLLDLPPSQ
KWLECLFVQGIPSRIYAIFLTEEDHYFDTIPTRDHFKWFYGFLAKKSPFDLKRYKEDLAIHKGWSKETIDFMAKVFFELE
FVTINNGLISLVKNSAKRDLIESATYRQKQQQIEIEKNFIYTSYVQLKNQFQDIFKRSIE

Nucleotide


Download         Length: 2343 bp        

>NTDB_id=845037 QRD24_RS23315 WP_286127763.1 4453638..4455980(-) (recJ) [Priestia megaterium strain ELP1.24a]
ATGTTACAGTCAAAAAAACGTTGGAATGTTCAAGAATGTGATGAACAACTTGTTAATGAATTTGTAGAAAACCTCAATAT
TACACCTCTAGTAGCTTCGTTATTACTTAATAGAGGCATGACAACAGTAGAAGCGGCGCGTGAGTTTTTACACGTTGATG
CACAAACGTTCCACGATCCGTTTTTGTTGCACGATATGGACAAAGCAGTAGCTCGTATTCAGCAAGCAATTGATCATAAT
GAAAAAATTATGATCTATGGAGATTACGATGCTGACGGCGTCAGCAGCACTTCTGTTATGTTATCTGCTTTACAGCAGCT
TGGCGCTGATGTTGATTTTTATATTCCTAATCGCTTTACAGAAGGTTATGGGCCGAATAAGGCGGCATTTGATAAAATAA
GCGCAGGCGGCTATCAGCTGGTGATTACGGTAGACACAGGTATTTCGGCAGTCGAAGAAACTGCGTATGCACGTGAATTA
AACTTTGATTTAATTATTACGGATCACCACGAGCCGGGGCCGGTTTTACCGGATGCTTATGCAATTATTCATCCAAAGCT
GCCGGGAAGTACATATCCATTTAAAGAACTGGCAGGTGTAGGAGTGGCTTTTAAACTCGCTCATGCGCTGTTAGGGGAGC
TTCCGGAACATCTGTTAGAACTAGCAGTAATTGGGACGATTGCTGACTTAGTTCCTTTAGTGGACGAAAACCGCTTAATT
GCTCGCAAAGGTCTTGATTATCTCAAGCTCACGAAGCGCACGGGAATTGAAGCGCTGCTGAGCGTATGCGGAGTAAAACG
TGAAGAAATTAATGAGGATACAATTGGATTTGCGATGGCTCCTCGCATTAATGCAGTAGGACGTCTTCAAGGTGCTGATC
CAGCTGTTCATTTACTTATGACGACAAATGCTGAAGAGGCAAAAGGTTTAGCTAAAGAAATTGATGCGTTAAACAAGGAA
AGACAGCAAATCGTAAACGATATTACAGAAGAAGCCGTTGAATTTGTTGAGAGTATGTATCCTCCGGATGAAAACAGCGT
TCTTATTGTAGAGGGAGAAGGATGGAATGCAGGCGTTGTTGGAATTGTCGCTTCTAGGCTCGTAGAAAAATTCTATAGAC
CAACAATTGTACTAAGTATTGACTCTGAAAACCGTATTGCCAAAGGTTCAGCAAGAAGTATCGAAGGTTTTGACTTATTC
GCGAACTTATCTAAGTGCCGTGACATTTTGCCGCATTTTGGAGGGCATCCAATGGCAGCCGGCATGACATTAAACAGCGA
TGATGTAGAAGAGCTGCGCAGACGTCTTAATGAACAAGCAAGCAACGTGTTGACCGAACAGGATTTTATTCCAATTACAT
CAGTTGATACAGTATGCACAACAGAGGAAATTACGCTGGAAAACATTGAACAAATGAGTGCGCTTGCTCCATTCGGTATG
CATAACCCGAAGCCAAAAGTTCTCATTAAAGATGTTCATACAACATCTATGCGGAAAATTGGTGCTAATAAAAATCATTT
AAAACTCGTATTTGAAGACAGCGGTGTATCGGTTGATGCAGTAGGATTTGGGTTAGGCTATATATTAGATGAAGTATCTC
CTATAGCTAAAGTATCATTAGTAGGTGAACTATCTATTAATGAGTGGAATAATATGCGCAAGCCGCAGCTGATGGTGGAA
GATATTTCTGTAGATGAATGGCAGATGTTTGATTATCGCAGTGCAGTTGATGTCAAACGTTTCTTTCAGCAGGTATTTAC
GGATCAAATGAAAGTTGTTGCTTTTCACGCTTCAACAATTGATAAATACAAGTCGCTGCTGCCGATGGAGCATGTTGTTC
TTATTCAAGATGAAGAACAGGCAAAAGCATGTATGTTTGCAGGGGAACAGATCGTTTTATTAGATTTGCCTCCTTCACAG
AAATGGCTTGAATGTTTGTTTGTCCAAGGTATCCCTTCTAGAATATACGCTATTTTCTTAACGGAAGAAGACCATTATTT
TGATACAATTCCAACTCGCGATCATTTTAAATGGTTTTACGGCTTTTTAGCGAAAAAGAGTCCATTTGATTTAAAACGCT
ACAAAGAAGATTTAGCGATTCATAAGGGCTGGTCAAAAGAAACGATTGATTTTATGGCAAAGGTGTTTTTTGAGTTAGAA
TTTGTTACAATAAACAATGGCTTAATTTCGTTAGTGAAAAATTCTGCGAAACGTGATTTAATAGAATCTGCGACTTATCG
CCAAAAGCAGCAGCAGATTGAAATAGAAAAAAACTTTATTTATACGTCATATGTACAGCTTAAAAATCAATTTCAAGATA
TTTTTAAGCGTTCGATTGAATGA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)

DHHA1 domain (DHHA1)

(305-439)

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(455-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.641

100

0.556