Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   V2201_RS09305 Genome accession   NZ_CP143897
Coordinates   2031425..2031850 (-) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain PA27_WM3_03.23_ST27     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2026425..2036850
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V2201_RS09285 (V2201_09285) ileS 2026582..2029413 (+) 2832 WP_003094730.1 isoleucine--tRNA ligase -
  V2201_RS09290 (V2201_09290) lspA 2029406..2029915 (+) 510 WP_003102615.1 signal peptidase II -
  V2201_RS09295 (V2201_09295) fkpB 2029908..2030348 (+) 441 WP_023114844.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  V2201_RS09300 (V2201_09300) ispH 2030434..2031378 (+) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  V2201_RS09305 (V2201_09305) comF 2031425..2031850 (-) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  V2201_RS09310 (V2201_09310) pilY2 2031847..2032194 (-) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  V2201_RS09315 (V2201_09315) pilY1 2032196..2035687 (-) 3492 WP_023114843.1 type 4a pilus biogenesis protein PilY1 -
  V2201_RS09320 (V2201_09320) pilX 2035699..2036286 (-) 588 WP_023114842.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=844652 V2201_RS09305 WP_003094721.1 2031425..2031850(-) (comF) [Pseudomonas aeruginosa strain PA27_WM3_03.23_ST27]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=844652 V2201_RS09305 WP_003094721.1 2031425..2031850(-) (comF) [Pseudomonas aeruginosa strain PA27_WM3_03.23_ST27]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCAGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGCTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383