Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   NSY31_RS05365 Genome accession   NZ_CP127834
Coordinates   1039389..1042889 (+) Length   1166 a.a.
NCBI ID   WP_098081271.1    Uniprot ID   -
Organism   Bacillus velezensis strain Ag109     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1034389..1047889
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSY31_RS05340 (NSY31_05340) - 1034550..1035707 (-) 1158 WP_015239535.1 AbrB family transcriptional regulator -
  NSY31_RS05345 (NSY31_05345) - 1035875..1037071 (-) 1197 WP_063095722.1 MFS transporter -
  NSY31_RS05350 (NSY31_05350) - 1037087..1037707 (-) 621 WP_012117247.1 TetR/AcrR family transcriptional regulator -
  NSY31_RS05355 (NSY31_05355) - 1037958..1038467 (-) 510 WP_007610501.1 ferritin-like domain-containing protein -
  NSY31_RS05360 (NSY31_05360) - 1038536..1039249 (-) 714 WP_020955605.1 YetF domain-containing protein -
  NSY31_RS05365 (NSY31_05365) addB 1039389..1042889 (+) 3501 WP_098081271.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  NSY31_RS05370 (NSY31_05370) addA 1042876..1046580 (+) 3705 WP_098081973.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  NSY31_RS05375 (NSY31_05375) sbcD 1046645..1047817 (+) 1173 WP_098081272.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 133401.93 Da        Isoelectric Point: 5.3565

>NTDB_id=844507 NSY31_RS05365 WP_098081271.1 1039389..1042889(+) (addB) [Bacillus velezensis strain Ag109]
MGVEFLVGRSGSGKTRLIIDSIQDELRREPFGKPIIFLVPDQMTFLMEYELAKTPDIGGMIRAQVFSFSRLAWRVLQHTG
GMNRPFVTTTGVQMLLRKLIEEHKHEFKVYQKASDKTGFTEQVERMLTEFKRYCIEPEDVRRMAESGTASEYRGERMLSE
KLHDLGILYQQMERSLAGHYLHSEDYLTLLAQQIPLADVVKGARVYVDGFYQFTPQELRVLEQLIMHAEHVTFSLTADSP
STEQAPDELDLFRMTGSTYYKLYQTAKELNADISCKELHGTKRHRHAPELACIESQYDVRPAAAYTGGQEAFTVMQAQNR
RAELEGIAREIQSLVRDGGYRYKDMAILIRQPEDYKDLLKEVFADYGLPYFIDGKASMQHHPLIEFIRSSLDVVKGNWRY
EAVFRCAKTELLFPLDQPEQKIREQVDQLENYCIAYGIKGERWTSGERFVYRRFVSLDEDFAQTDQEIEMEHMLNETKEW
MAAPLVKLQNRMKKAKTVQSMAEALYLFLEDTDVPLKLDRKRQRAEEAGNMIEAQQHGQAWDAVIQLLEEFAGMMGEDEI
SLALFQQMLETGTESLHFSLIPPALDQVFVGNMDLSRMYGTSCTFVIGANDGVLPARPDENGVLSDDDREWLKAVGVELS
SAGRERLLDEHFLIYMALSSPSDRLYVSYPIADAEGKTLLPSIVVNRLSELFPDHQEKLSAADPEQVSEEEQLQYLVNKQ
VAQTYTASQLRLWTREYEISDVWWSAYNVLMKEPDHQRAKKLFSSLFFRNEAKRLERPVSRQLYGEHIKGSVSRMEAFNA
CQFSHFASHGLQLKERQFFKLDAPDIGQLFHSSLKLISDRLREQKLEWRDLTKDQCRNFSYEAVERLAPKLQKEILLSSN
RHFYVKEKLQKIVTRVSGILSEHAKASGFVPVGLELGFGGSGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDRGLDLAEVYYGLALQMLTYLDLSITHSEDWLGMKATPAGVLYFHIHDPMIQASLPMGLDEIEQEIFKKFKMKGL
LLGDREAISLMDTTLEEGRSNIVNAGLKKDGSLRSDSAAVSERDFHLLTDHVRRTFEQAGEAITDGLVSITPYKLKDKTP
CTYCAFQSVCQFDESLKENEYRSLKAEKDGTILDWLKKEADDDANS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=844507 NSY31_RS05365 WP_098081271.1 1039389..1042889(+) (addB) [Bacillus velezensis strain Ag109]
TTGGGTGTTGAGTTTTTAGTAGGCAGATCGGGGAGTGGGAAAACGAGGCTGATTATCGACAGCATTCAAGATGAACTGCG
CCGGGAGCCGTTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCGGATATCGGCGGCATGATACGCGCTCAAGTGTTCAGTTTTTCGCGTTTGGCGTGGCGCGTGCTTCAGCATACCGGG
GGCATGAACCGCCCGTTTGTGACGACGACGGGTGTGCAGATGCTTTTAAGGAAATTGATTGAAGAACATAAGCACGAATT
TAAAGTGTATCAAAAAGCGAGTGACAAAACCGGGTTCACTGAGCAGGTGGAACGGATGCTGACAGAGTTTAAGCGGTACT
GTATTGAGCCTGAAGACGTCCGCCGCATGGCGGAAAGCGGAACGGCGTCAGAATATCGCGGAGAGCGGATGCTGTCTGAA
AAACTTCATGATTTAGGCATCTTATATCAGCAGATGGAAAGAAGCCTCGCCGGACACTATTTGCATTCAGAGGATTATTT
GACGCTGCTCGCTCAGCAGATTCCTTTGGCGGACGTTGTAAAAGGCGCCCGTGTTTACGTCGACGGATTCTATCAATTTA
CGCCGCAGGAATTAAGGGTGCTGGAGCAGCTGATCATGCATGCTGAACATGTCACATTCTCGCTGACGGCGGACAGCCCT
TCGACGGAACAGGCACCGGATGAGCTCGATCTGTTCAGAATGACGGGAAGCACATACTATAAGCTTTATCAGACGGCAAA
GGAATTGAATGCCGATATTTCCTGCAAGGAACTGCATGGCACGAAGCGGCATCGGCATGCGCCGGAGCTTGCCTGTATAG
AGTCGCAGTACGATGTGCGTCCCGCCGCAGCGTATACCGGCGGACAGGAAGCTTTTACCGTCATGCAGGCGCAAAACAGA
AGAGCTGAGCTTGAAGGTATTGCCCGGGAAATTCAATCACTTGTCCGGGATGGAGGATACCGGTACAAAGATATGGCGAT
TCTCATCCGTCAGCCGGAAGATTATAAAGATTTGCTGAAGGAAGTGTTTGCCGATTACGGGCTTCCTTATTTTATTGACG
GAAAAGCTTCTATGCAGCATCACCCGCTTATTGAATTCATCCGTTCAAGCCTGGATGTCGTGAAGGGGAACTGGAGATAT
GAAGCGGTGTTCCGCTGTGCGAAAACCGAACTGTTATTCCCGCTTGATCAGCCGGAGCAGAAAATAAGGGAGCAGGTTGA
TCAGCTTGAAAATTACTGTATCGCCTACGGCATTAAAGGGGAGCGCTGGACGAGCGGCGAGCGGTTTGTATACAGGCGCT
TCGTATCGCTCGATGAGGATTTCGCGCAGACGGATCAGGAAATAGAGATGGAACACATGCTGAATGAGACGAAAGAGTGG
ATGGCCGCGCCCCTCGTCAAGCTCCAAAACCGGATGAAAAAGGCGAAAACGGTTCAAAGCATGGCGGAGGCTCTTTATCT
CTTTTTGGAAGATACCGATGTTCCCCTGAAGCTGGACCGGAAAAGGCAGCGGGCCGAAGAGGCCGGAAACATGATCGAAG
CGCAGCAGCACGGCCAGGCGTGGGATGCCGTTATCCAGCTTCTCGAGGAGTTTGCCGGCATGATGGGTGAAGATGAAATA
TCTTTGGCGCTGTTCCAGCAAATGCTTGAAACGGGAACGGAATCGCTTCATTTTTCTTTAATTCCGCCTGCGCTTGACCA
AGTATTTGTAGGGAATATGGATCTCTCAAGAATGTACGGCACGTCCTGCACATTCGTCATCGGGGCCAATGACGGCGTTC
TTCCGGCGCGCCCGGATGAAAACGGCGTGCTGTCTGATGATGACCGGGAGTGGCTGAAAGCAGTCGGAGTGGAGCTGTCT
TCCGCGGGGCGTGAGCGGCTGCTTGATGAGCATTTTCTCATCTATATGGCGCTGTCAAGTCCGTCTGACCGTCTTTATGT
GTCATACCCGATTGCCGATGCGGAAGGAAAAACGCTCCTGCCGTCAATCGTTGTAAACAGGCTTAGCGAGCTGTTTCCCG
ATCATCAGGAAAAGCTGTCAGCGGCTGATCCCGAGCAGGTAAGCGAAGAAGAGCAGCTTCAGTATCTCGTCAATAAACAG
GTGGCTCAGACATATACGGCAAGTCAGCTCAGGCTGTGGACAAGAGAGTACGAGATCAGTGACGTATGGTGGAGCGCATA
CAATGTGCTGATGAAAGAGCCTGATCACCAGCGTGCAAAAAAGCTGTTTTCAAGCTTGTTTTTCCGCAATGAGGCGAAGC
GTCTTGAACGGCCTGTTTCGCGCCAGCTTTACGGCGAGCATATTAAAGGAAGCGTCTCCCGGATGGAGGCGTTTAATGCC
TGCCAGTTCTCTCATTTTGCATCTCACGGTCTTCAATTAAAAGAGCGACAGTTTTTCAAGCTGGACGCGCCGGATATCGG
CCAGCTGTTTCACTCCAGCCTGAAGCTGATTTCAGACCGGCTACGTGAGCAGAAGCTGGAATGGCGCGATCTGACGAAAG
ATCAGTGCCGGAATTTCTCCTACGAAGCGGTGGAACGGCTTGCGCCAAAGCTGCAAAAAGAGATTCTGCTCAGCTCAAAC
AGACACTTTTATGTGAAGGAAAAACTGCAGAAGATCGTCACGCGTGTTTCTGGCATATTAAGCGAGCATGCGAAGGCGAG
CGGTTTCGTCCCGGTCGGTCTTGAACTCGGTTTCGGCGGAAGCGGGCCGCTGCCTCCGCTCACTTTCACGCTGAAAAACG
GCTGTACGATGGAACTCGTCGGACGGATCGACCGCGTGGATAAAGCGGAAAGTTCAAAAGGCCTGCTGCTGAGAATCGTT
GATTATAAATCAAGCGACAGGGGGCTTGATCTGGCTGAGGTGTATTACGGGCTGGCTCTGCAAATGCTCACATATCTTGA
CTTATCGATTACACATTCCGAAGATTGGCTCGGCATGAAAGCGACCCCGGCAGGCGTTTTGTATTTCCATATCCACGATC
CGATGATTCAGGCCAGTCTGCCGATGGGGCTTGATGAAATCGAGCAGGAAATTTTCAAGAAATTTAAAATGAAAGGCCTT
CTCCTCGGTGACCGGGAGGCGATCAGCCTGATGGACACGACTTTGGAGGAAGGGCGTTCAAATATCGTCAATGCCGGTTT
GAAAAAGGACGGTTCTTTAAGATCCGACTCAGCCGCGGTCAGCGAACGGGATTTTCATCTGCTGACAGACCATGTGCGGC
GGACATTTGAACAGGCGGGCGAAGCGATAACGGACGGGCTTGTGTCCATTACTCCGTATAAGCTGAAAGATAAGACGCCT
TGTACGTACTGTGCGTTTCAATCGGTGTGCCAGTTTGATGAGTCGCTTAAGGAAAATGAATACCGCAGCCTGAAAGCGGA
AAAAGACGGCACCATACTGGATTGGCTGAAGAAGGAGGCGGATGATGATGCAAATTCCTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(791-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

83.276

100

0.833