Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   VYI50_RS02050 Genome accession   NZ_CP142956
Coordinates   456066..456533 (-) Length   155 a.a.
NCBI ID   WP_022646190.1    Uniprot ID   -
Organism   Escherichia coli strain EC200461     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 451066..461533
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VYI50_RS02025 (VYI50_02025) rpsG 451207..451677 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  VYI50_RS02030 (VYI50_02030) fusA 451774..453888 (+) 2115 WP_000124700.1 elongation factor G -
  VYI50_RS02035 (VYI50_02035) tuf 453959..455143 (+) 1185 WP_000031783.1 elongation factor Tu -
  VYI50_RS02040 (VYI50_02040) bfd 455326..455520 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  VYI50_RS02045 (VYI50_02045) bfr 455593..456069 (+) 477 WP_000675515.1 bacterioferritin -
  VYI50_RS02050 (VYI50_02050) pilD 456066..456533 (-) 468 WP_022646190.1 prepilin peptidase Machinery gene
  VYI50_RS02055 (VYI50_02055) rpsJ 456912..457223 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  VYI50_RS02060 (VYI50_02060) rplC 457256..457885 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  VYI50_RS02065 (VYI50_02065) rplD 457896..458501 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  VYI50_RS02070 (VYI50_02070) rplW 458498..458800 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  VYI50_RS02075 (VYI50_02075) rplB 458818..459639 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  VYI50_RS02080 (VYI50_02080) rpsS 459656..459934 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  VYI50_RS02085 (VYI50_02085) rplV 459949..460281 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  VYI50_RS02090 (VYI50_02090) rpsC 460299..461000 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  VYI50_RS02095 (VYI50_02095) rplP 461013..461423 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17064.20 Da        Isoelectric Point: 8.1255

>NTDB_id=843121 VYI50_RS02050 WP_022646190.1 456066..456533(-) (pilD) [Escherichia coli strain EC200461]
MDATLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLATAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=843121 VYI50_RS02050 WP_022646190.1 456066..456533(-) (pilD) [Escherichia coli strain EC200461]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCAGCCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACGTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAAGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATTCTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACATGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGACTGCGGGTTTCGTTGTAGGCTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(11-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.882

81.935

0.368