Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   VVK77_RS01980 Genome accession   NZ_CP142710
Coordinates   298897..299661 (-) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain T16R     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 293897..304661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VVK77_RS01970 (VVK77_01970) nikR 297687..298088 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  VVK77_RS01975 (VVK77_01975) nikE 298094..298900 (-) 807 WP_000173631.1 nickel import ATP-binding protein NikE -
  VVK77_RS01980 (VVK77_01980) amiE 298897..299661 (-) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  VVK77_RS01985 (VVK77_01985) nikC 299661..300494 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  VVK77_RS01990 (VVK77_01990) nikB 300491..301435 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  VVK77_RS01995 (VVK77_01995) nikA 301435..303009 (-) 1575 WP_000953361.1 nickel ABC transporter substrate-binding protein -
  VVK77_RS02000 (VVK77_02000) acpT 303120..303707 (-) 588 WP_000285774.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=841783 VVK77_RS01980 WP_001136229.1 298897..299661(-) (amiE) [Escherichia coli strain T16R]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=841783 VVK77_RS01980 WP_001136229.1 298897..299661(-) (amiE) [Escherichia coli strain T16R]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCTCCCAAACATACAGTGACACGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398