Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   VT654_RS01295 Genome accession   NZ_CP142590
Coordinates   280940..281704 (-) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli strain 64     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 275940..286704
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VT654_RS01270 (VT654_01270) yhhJ 277796..278920 (+) 1125 WP_001216257.1 ABC transporter permease -
  VT654_RS01275 (VT654_01275) - 279024..279254 (+) 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin -
  VT654_RS01280 (VT654_01280) - 279251..279610 (+) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  VT654_RS01285 (VT654_01285) nikR 279730..280131 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  VT654_RS01290 (VT654_01290) nikE 280137..280943 (-) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  VT654_RS01295 (VT654_01295) amiE 280940..281704 (-) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  VT654_RS01300 (VT654_01300) nikC 281704..282537 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  VT654_RS01305 (VT654_01305) nikB 282534..283478 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  VT654_RS01310 (VT654_01310) nikA 283478..285052 (-) 1575 WP_000953353.1 nickel ABC transporter substrate-binding protein -
  VT654_RS01315 (VT654_01315) acpT 285163..285750 (-) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=841277 VT654_RS01295 WP_001136236.1 280940..281704(-) (amiE) [Escherichia coli strain 64]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=841277 VT654_RS01295 WP_001136236.1 280940..281704(-) (amiE) [Escherichia coli strain 64]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398