Detailed information NTG0841241

Bioinformatically predicted

Overview

• Name: endA
• Type: Machinery gene
• Locus tag: VVB72_RS09510
• Genome accession: NZ_CP142589
• Coordinates: 1928439..1929218 (-)
• Length: 259 a.a.
• NCBI ID: WP_050428201.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain C20
• Function: cleavage of dsDNA into ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1923439..1934218

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VVB72_RS12135	-	1924435..1924633 (-)	199	Protein_1845	hypothetical protein	-
VVB72_RS09485 (VVB72_09485)	-	1924630..1924830 (-)	201	WP_039115873.1	DUF1655 domain-containing protein	-
VVB72_RS09490 (VVB72_09490)	-	1925178..1925354 (-)	177	WP_039115875.1	helix-turn-helix domain-containing protein	-
VVB72_RS09495 (VVB72_09495)	-	1925499..1926473 (+)	975	WP_039115877.1	helix-turn-helix domain-containing protein	-
VVB72_RS09500 (VVB72_09500)	-	1926553..1927752 (+)	1200	WP_039115879.1	tyrosine-type recombinase/integrase	-
VVB72_RS09505 (VVB72_09505)	-	1927899..1928348 (+)	450	WP_025017176.1	GNAT family N-acetyltransferase	-
VVB72_RS09510 (VVB72_09510)	endA	1928439..1929218 (-)	780	WP_050428201.1	DNA/RNA non-specific endonuclease	Machinery gene
VVB72_RS09515 (VVB72_09515)	-	1929412..1930308 (-)	897	WP_039115881.1	peptidoglycan amidohydrolase family protein	-
VVB72_RS09520 (VVB72_09520)	-	1930420..1930674 (-)	255	WP_023189062.1	phage holin	-
VVB72_RS09525 (VVB72_09525)	-	1930664..1930966 (-)	303	WP_025017161.1	hypothetical protein	-
VVB72_RS09530 (VVB72_09530)	-	1931171..1931464 (+)	294	WP_025017162.1	hypothetical protein	-
VVB72_RS09535 (VVB72_09535)	-	1931494..1933734 (-)	2241	WP_052230930.1	glycoside hydrolase domain-containing protein	-
VVB72_RS09540 (VVB72_09540)	-	1933834..1934181 (-)	348	WP_023189066.1	DUF722 domain-containing protein	-

Sequence

Protein

• Length: 259 a.a.
• Molecular weight: 29129.05 Da
• Isoelectric Point: 9.0583

>NTDB_id=841241 VVB72_RS09510 WP_050428201.1 1928439..1929218(-) (endA) [Lactococcus lactis subsp. lactis strain C20]
MKNKKIIPLLIVSIFILGGCANSQSDMDKSNHKWSQKDKTPSEELAKSVLTSSIKEQLKTDNIKFDHGSFIVNNNNNDLA
IINVVDPYAKNEIDIQGRPYLGEAVLPKSIQNNEKDNKDEKVIPQFGWGQKSNLKGFYKKAYTKGFLLNSKLIGGVKDFN
DSGSNMENIFTQTVWASEANDSSSKGQKYFENMIHKVQDKNMIIKYRVKALYDGSNLVPSGTEIEAKSDDFSLEIHVFIP
NVQPNLKVDYKTGQVTEVK

Nucleotide

• Length: 780 bp

>NTDB_id=841241 VVB72_RS09510 WP_050428201.1 1928439..1929218(-) (endA) [Lactococcus lactis subsp. lactis strain C20]
ATGAAGAACAAAAAAATTATCCCATTACTGATTGTCAGCATTTTTATCTTAGGGGGCTGTGCTAACAGTCAATCTGATAT
GGATAAAAGTAATCATAAATGGAGTCAAAAAGATAAGACACCCTCAGAAGAACTCGCAAAAAGTGTTCTGACTTCTTCAA
TAAAAGAACAATTAAAAACAGATAACATAAAATTTGATCACGGTTCATTTATCGTTAATAATAATAACAATGATTTAGCA
ATTATTAATGTTGTAGACCCATACGCTAAGAATGAAATAGATATTCAAGGGCGTCCTTATTTGGGTGAAGCAGTTCTCCC
AAAATCTATTCAAAATAATGAAAAGGATAATAAAGATGAAAAAGTGATTCCTCAATTTGGTTGGGGACAAAAATCTAACT
TGAAAGGCTTCTATAAAAAAGCTTATACTAAAGGTTTTTTGCTAAATAGCAAACTAATAGGTGGCGTTAAAGATTTCAAT
GATAGCGGGTCAAATATGGAAAATATATTTACTCAAACGGTTTGGGCGAGTGAAGCTAATGATTCAAGTTCCAAGGGACA
AAAATATTTTGAAAATATGATTCATAAAGTTCAAGATAAGAATATGATCATCAAGTATAGAGTGAAAGCACTCTATGATG
GAAGTAACCTTGTCCCATCAGGAACTGAAATAGAAGCAAAATCTGATGATTTTTCACTCGAGATACATGTTTTTATTCCA
AATGTTCAACCAAATCTTAAAGTAGATTATAAAACAGGTCAGGTCACTGAAGTCAAATAA

Domains

Predicted by InterProScan.

• DNA/RNA non-specific endonuclease (Endonuclea_NS_2) (92-229)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
endA	Streptococcus pneumoniae Rx1	42.174	88.803	0.375
endA	Streptococcus pneumoniae D39	42.174	88.803	0.375
endA	Streptococcus pneumoniae R6	42.174	88.803	0.375
endA	Streptococcus pneumoniae TIGR4	42.174	88.803	0.375