Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   VJW35_RS01235 Genome accession   NZ_CP142382
Coordinates   269548..270312 (-) Length   254 a.a.
NCBI ID   WP_327442505.1    Uniprot ID   -
Organism   Escherichia coli strain HS1496     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 264548..275312
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VJW35_RS01205 (VJW35_01205) - 265182..266540 (-) 1359 WP_001525857.1 PTS galactitol transporter subunit IIC -
  VJW35_RS01210 (VJW35_01210) - 266617..266898 (-) 282 WP_000084021.1 PTS sugar transporter subunit IIB -
  VJW35_RS01215 (VJW35_01215) - 266895..267368 (-) 474 WP_001161648.1 PTS sugar transporter subunit IIA -
  VJW35_RS01220 (VJW35_01220) - 267393..268139 (-) 747 WP_001296489.1 GntR family transcriptional regulator -
  VJW35_RS01225 (VJW35_01225) nikR 268338..268739 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  VJW35_RS01230 (VJW35_01230) nikE 268745..269551 (-) 807 WP_000173684.1 nickel import ATP-binding protein NikE -
  VJW35_RS01235 (VJW35_01235) amiE 269548..270312 (-) 765 WP_327442505.1 nickel import ATP-binding protein NikD Regulator
  VJW35_RS01240 (VJW35_01240) nikC 270312..271145 (-) 834 WP_001008954.1 nickel ABC transporter permease subunit NikC -
  VJW35_RS01245 (VJW35_01245) nikB 271142..272086 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  VJW35_RS01250 (VJW35_01250) nikA 272086..273660 (-) 1575 WP_000493124.1 nickel ABC transporter substrate-binding protein -
  VJW35_RS01255 (VJW35_01255) acpT 273771..274358 (-) 588 WP_000285760.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26842.43 Da        Isoelectric Point: 6.6882

>NTDB_id=840942 VJW35_RS01235 WP_327442505.1 269548..270312(-) (amiE) [Escherichia coli strain HS1496]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVSGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=840942 VJW35_RS01235 WP_327442505.1 269548..270312(-) (amiE) [Escherichia coli strain HS1496]
ATGCCACAACAGATTGAACTCCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGTGGGCGTGTGCTGGCGTTAGTCAGCGGTAGTGGCAGCGGGAAGTCGCTGACCTGCGCCGCGACGCTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTGGCCGATGGAAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCAACCACCGATCTCGACGTGGTAGCACAAGCACGCATCCTCGATCTACTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTAGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCGCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.2

98.425

0.406
  amiE Streptococcus thermophilus LMD-9

41.2

98.425

0.406
  amiE Streptococcus salivarius strain HSISS4

40

98.425

0.394