Detailed information NTG0838575

Bioinformatically predicted

Overview

• Name: addB
• Type: Machinery gene
• Locus tag: QN218_RS12925
• Genome accession: NZ_CP126534
• Coordinates: 2472642..2476142 (-)
• Length: 1166 a.a.
• NCBI ID: WP_060398327.1
• Uniprot ID: -
• Organism: Bacillus inaquosorum strain BSY82
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 2467642..2481142

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QN218_RS12915	sbcD	2467704..2468879 (-)	1176	WP_060398329.1	exonuclease subunit SbcD	-
QN218_RS12920	addA	2468951..2472655 (-)	3705	WP_060398328.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
QN218_RS12925	addB	2472642..2476142 (-)	3501	WP_060398327.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
QN218_RS12930	-	2476325..2476762 (+)	438	WP_044427329.1	ferritin family protein	-
QN218_RS12935	-	2476879..2477208 (+)	330	WP_060398326.1	four-helix bundle copper-binding protein	-
QN218_RS12940	-	2477245..2478972 (-)	1728	WP_060398325.1	ABC transporter substrate-binding protein	-
QN218_RS12945	-	2479086..2480288 (+)	1203	WP_060398324.1	MFS transporter	-

Sequence

Protein

• Length: 1166 a.a.
• Molecular weight: 134654.76 Da
• Isoelectric Point: 5.6870

>NTDB_id=838575 QN218_RS12925 WP_060398327.1 2472642..2476142(-) (addB) [Bacillus inaquosorum strain BSY82]
MGAEFLVGRSGSGKTKLIIDSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDMGGMIRAQVFSFSRLAWRVLQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMVESGTASEYRGERILSE
KLHDLSILYQQMEKSLAGQYLHSEDYLTLLAEHVPLAEDVKGAHIYVDGFYQFTPQEFRVLEQLMVHAEHITFSLTADKP
SYEREPHELELFRMTGKTYYRLHQKAKELNLDITYKELSGTERHTRTPELAHLEAQYEARPAVPYAEKQEAFTVMQAANR
RAELEGIAREIHALVREKGYRYKDVAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSLDVLKGNWRY
EAVFRCVKTELLFPLKEPKTKVREQVDQLENYCIAYGIKGDRWTKGDRFHYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVQEKAEALYRYLEETDVPLKLDQERQRAEEDGRIIEAQQHQQAWDAVIQLLEEFVEMMGDDEI
SLDLFQQMMEAGAESLTFSLIPPALDQVFVGNMDLSRMYGTSCTFVLGANDGVLPARPDENGVLSDDDREWLKTVGVELS
SGGRERLLDEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPSIVIKRLEELFPQHKERLLTNEPEQVSDEEQLMYVVNKS
VAQSFTASQLRLWTREYDISDVWWSTYNVLMSEPDRLQSKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKMDWRDLTKEQCELFSHDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFKLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEAIRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFELLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRPLKAEKDKTILEWIKKEADGNEHS

Nucleotide

• Length: 3501 bp

>NTDB_id=838575 QN218_RS12925 WP_060398327.1 2472642..2476142(-) (addB) [Bacillus inaquosorum strain BSY82]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCGACAGCATTCAGGATGAACTGCG
CCGGGCTCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCTGATATGGGCGGAATGATACGCGCTCAAGTGTTCAGCTTTTCACGATTGGCCTGGCGCGTCCTCCAGCATACGGGA
GGCATGAGCAGGCCGTTTCTTACGAGCACAGGCGTACAGATGCTCCTGCGGAAGCTCATTGAGGAGCATAAACAGGAGTT
TAAAGTCTATCAAAAAGCGAGTGACAAAAGCGGGTTTACCGCACAGGTAGAACGGATGCTGACAGAGTTTAAGCGCTACT
GTCTGGAACCGGAAGACATCCGCCGGATGGTGGAAAGCGGAACGGCTTCCGAGTATCGCGGAGAACGTATTTTATCTGAG
AAGCTTCATGATTTATCGATTTTATATCAACAGATGGAAAAAAGCCTTGCAGGTCAATACCTTCACTCTGAGGATTATTT
AACATTGCTGGCAGAGCATGTTCCGCTTGCAGAAGATGTAAAAGGCGCGCATATCTATGTGGACGGCTTTTATCAGTTTA
CACCGCAGGAATTCAGGGTGTTGGAGCAGCTTATGGTTCATGCAGAGCACATCACGTTTTCGCTGACAGCGGACAAGCCG
TCATATGAGCGGGAGCCGCATGAACTGGAATTGTTCAGAATGACGGGAAAAACCTATTACCGCCTGCACCAAAAGGCGAA
GGAACTGAATCTGGACATTACGTATAAAGAGCTAAGCGGGACTGAACGGCATACGCGCACACCGGAATTGGCACATTTAG
AGGCGCAGTATGAAGCGCGTCCAGCCGTTCCGTACGCAGAAAAACAAGAGGCCTTTACTGTGATGCAGGCCGCAAACAGA
CGAGCTGAGCTGGAAGGCATTGCCCGGGAAATTCACGCTCTTGTCAGAGAGAAGGGCTATCGCTATAAGGATGTAGCGAT
TCTTGCGCGCCAGCCGGAAGACTACAAGGATATGGTGAAGGAAGTTTTCGCAGATTACGAGATTCCTTATTTCATTGACG
GAAAAGCATCAATGCTGAACCATCCGTTAATCGAATTTATCCGGTCGAGCCTTGATGTTTTGAAAGGGAATTGGCGTTAT
GAAGCAGTGTTTCGCTGCGTGAAAACCGAACTGCTATTCCCGCTTAAAGAGCCGAAGACTAAGGTGAGAGAACAGGTCGA
TCAGCTTGAAAATTACTGTATCGCCTACGGGATCAAAGGTGACCGCTGGACAAAGGGCGACCGTTTCCATTACAGACGTT
TTGTGTCATTGGATGATGATTTTGCGCAGACCGACCAGGAAATCGAAATGGAAAACATGCTGAATGACACACGCGACTGG
ATTGTTCCTCCGCTTTTTCAGCTCCAGAAACGAATGAAAAAAGCAAAGACGGTTCAGGAGAAGGCGGAGGCGCTCTACCG
CTATCTAGAAGAGACGGATGTGCCGCTGAAGCTGGATCAGGAAAGACAGCGTGCAGAGGAAGACGGCAGAATCATTGAAG
CACAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTGCTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATT
TCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCAGAGTCGCTTACATTTTCTCTTATTCCGCCTGCGCTTGACCA
GGTGTTTGTCGGCAATATGGATTTGTCCAGAATGTATGGCACCTCCTGTACCTTTGTGCTGGGGGCAAACGACGGCGTTC
TGCCGGCGCGCCCAGATGAAAACGGAGTGCTGTCGGATGATGACCGGGAATGGCTGAAAACGGTTGGGGTTGAGCTTTCC
TCAGGCGGGCGGGAGCGTCTGCTTGATGAGCATTTCCTCATCTATATGGCGTTTTCCAGTCCGTCTGACCGGCTGTACGT
ATCGTATCCGATTGCTGATGCGGAAGGAAAAACGCTTCTGCCGTCGATTGTCATTAAGCGGCTGGAAGAACTGTTTCCGC
AGCATAAGGAGCGCCTGTTGACAAATGAACCTGAGCAGGTCAGCGATGAGGAACAGCTGATGTATGTCGTCAATAAAAGC
GTGGCACAGTCCTTTACCGCGAGCCAGCTCAGGTTATGGACTCGGGAATACGACATCAGCGACGTCTGGTGGAGCACGTA
CAATGTGTTGATGAGTGAGCCGGACAGGCTGCAATCGAAAAAGTTATTCTCAAGCCTGTTTTTCCGGAATGAAGTGAAGC
AGCTTGAACGCAGCGTGTCGAGACAGCTCTATGGTGAACGCATTCAGGGCAGTGTATCGAGAATGGAAACCTTTAACGCG
TGCCCGTTCTCCCATTTTGCGTCACACGGGCTGCATCTGAAGGAACGGCAATTTTTCAAGCTCGAAGCACCGGATATCGG
CCAGCTGTTTCATTCCAGCTTAAAGCTGATTTCAGACCGGCTGCGCGAGCAAAAAATGGATTGGCGCGATTTAACGAAAG
AGCAGTGCGAGCTGTTCTCCCATGATGCGGTAGAGCGTCTGGCGCCGAAACTGCAAAAGGAAATTTTGCTCAGCTCAAAC
CGGCATTATTATGTGAAGGAAAAACTGCAAAAAATTGTGACACGCGTGTCCGGCATTTTAAGCGAGCATGCGAAAGCGAG
CGGTTTCGTGCCGATCGGGCTTGAACTGGGCTTTGGAGGAAAAGGGCCGCTTCCGCCGCTGACCTTTAAGCTGAAAAACG
GCTGCACGATGGAGCTCGTCGGGCGAATTGACCGCGTAGATAAAGCGGAAAGCTCAAAAGGCCTGCTTCTAAGAATTGTC
GATTATAAATCAAGCGACAAAGGCCTTGACTTAGCGGAAGTATATTACGGACTGGCGCTGCAAATGCTGACGTACCTTGA
TTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGAGCGACGCCGGCCGGAGTGCTGTATTTCCATATTCATGACC
CGATGATTCAATCTAACCTCCCGCTTGGGCTTGACGAGATCGAACAGGAGATCTTTAAGAAATTTAAGATGAAGGGCCTG
CTCCTCGGCGATCAGGAAGCGATTCGCCTCATGGATACGACTCTTCAAGAGGGACGGTCAAATATCATCAACGCCGGCTT
GAAAAAAGACGGCTCTCTCAGATCGGACTCGGCGGCAGTCGGCGAAAAGGAATTTGAGCTGCTGACGAAGCATGTGCGCC
GCACCTTCCAAGAAGCAGGCGAACAAATCACCGACGGACGCGTATCGATTGAGCCTTATAAAATGAAGAACAAGACACCG
TGCACATACTGTGCGTTCAAATCAGTATGCCAATTTGATGAATCATTAGAAGAAAACGAGTATCGCCCGTTAAAGGCTGA
AAAGGACAAGACGATACTTGAGTGGATAAAAAAGGAGGCGGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (306-615)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (790-1131)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addB	Bacillus subtilis subsp. subtilis str. 168	97.77	100	0.978