Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   VB129_RS01260 Genome accession   NZ_CP141844
Coordinates   275730..276494 (-) Length   254 a.a.
NCBI ID   WP_097412446.1    Uniprot ID   -
Organism   Escherichia coli strain HN68     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 270730..281494
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VB129_RS01235 (VB129_01235) yhhJ 272586..273710 (+) 1125 WP_001314210.1 ABC transporter permease -
  VB129_RS01240 (VB129_01240) - 273814..274044 (+) 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin -
  VB129_RS01245 (VB129_01245) - 274041..274400 (+) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  VB129_RS01250 (VB129_01250) nikR 274520..274921 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  VB129_RS01255 (VB129_01255) nikE 274927..275733 (-) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  VB129_RS01260 (VB129_01260) amiE 275730..276494 (-) 765 WP_097412446.1 nickel import ATP-binding protein NikD Regulator
  VB129_RS01265 (VB129_01265) nikC 276494..277327 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  VB129_RS01270 (VB129_01270) nikB 277324..278268 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  VB129_RS01275 (VB129_01275) nikA 278268..279842 (-) 1575 WP_000953356.1 nickel ABC transporter substrate-binding protein -
  VB129_RS01280 (VB129_01280) acpT 279953..280540 (-) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26832.43 Da        Isoelectric Point: 6.8308

>NTDB_id=838501 VB129_RS01260 WP_097412446.1 275730..276494(-) (amiE) [Escherichia coli strain HN68]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPANDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=838501 VB129_RS01260 WP_097412446.1 275730..276494(-) (amiE) [Escherichia coli strain HN68]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCAATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398