Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   U8328_RS02050 Genome accession   NZ_CP141767
Coordinates   452085..452552 (-) Length   155 a.a.
NCBI ID   WP_000338336.1    Uniprot ID   -
Organism   Escherichia coli strain T-HYM1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 447085..457552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U8328_RS02025 (U8328_02025) rpsG 447226..447696 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  U8328_RS02030 (U8328_02030) fusA 447793..449907 (+) 2115 WP_000124703.1 elongation factor G -
  U8328_RS02035 (U8328_02035) tuf 449978..451162 (+) 1185 WP_000031783.1 elongation factor Tu -
  U8328_RS02040 (U8328_02040) bfd 451345..451539 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  U8328_RS02045 (U8328_02045) bfr 451612..452088 (+) 477 WP_000675515.1 bacterioferritin -
  U8328_RS02050 (U8328_02050) pilD 452085..452552 (-) 468 WP_000338336.1 prepilin peptidase Machinery gene
  U8328_RS02055 (U8328_02055) rpsJ 452931..453242 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  U8328_RS02060 (U8328_02060) rplC 453275..453904 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  U8328_RS02065 (U8328_02065) rplD 453915..454520 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  U8328_RS02070 (U8328_02070) rplW 454517..454819 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  U8328_RS02075 (U8328_02075) rplB 454837..455658 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  U8328_RS02080 (U8328_02080) rpsS 455675..455953 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  U8328_RS02085 (U8328_02085) rplV 455968..456300 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  U8328_RS02090 (U8328_02090) rpsC 456318..457019 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  U8328_RS02095 (U8328_02095) rplP 457032..457442 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17034.17 Da        Isoelectric Point: 8.1255

>NTDB_id=837604 U8328_RS02050 WP_000338336.1 452085..452552(-) (pilD) [Escherichia coli strain T-HYM1]
MDAALPFLILYACLSTLLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=837604 U8328_RS02050 WP_000338336.1 452085..452552(-) (pilD) [Escherichia coli strain T-HYM1]
ATGGACGCTGCCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCAACCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGGTTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAAGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGCCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACATGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTTTGCTTATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGTCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGCTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.882

81.935

0.368