Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   U8328_RS01305 Genome accession   NZ_CP141767
Coordinates   296753..297517 (-) Length   254 a.a.
NCBI ID   WP_001136198.1    Uniprot ID   -
Organism   Escherichia coli strain T-HYM1     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 291753..302517
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U8328_RS01280 (U8328_01280) yhhJ 293596..294720 (+) 1125 WP_001315056.1 ABC transporter permease -
  U8328_RS01285 (U8328_01285) - 294793..295068 (+) 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin -
  U8328_RS01290 (U8328_01290) - 295065..295424 (+) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  U8328_RS01295 (U8328_01295) nikR 295544..295945 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  U8328_RS01300 (U8328_01300) nikE 295950..296756 (-) 807 WP_000173658.1 nickel import ATP-binding protein NikE -
  U8328_RS01305 (U8328_01305) amiE 296753..297517 (-) 765 WP_001136198.1 nickel import ATP-binding protein NikD Regulator
  U8328_RS01310 (U8328_01310) nikC 297517..298350 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  U8328_RS01315 (U8328_01315) nikB 298347..299291 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  U8328_RS01320 (U8328_01320) nikA 299291..300865 (-) 1575 WP_000953379.1 nickel ABC transporter substrate-binding protein -
  U8328_RS01325 (U8328_01325) acpT 300976..301563 (-) 588 WP_000285778.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26843.46 Da        Isoelectric Point: 6.6886

>NTDB_id=837599 U8328_RS01305 WP_001136198.1 296753..297517(-) (amiE) [Escherichia coli strain T-HYM1]
MPQQIELRDIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=837599 U8328_RS01305 WP_001136198.1 296753..297517(-) (amiE) [Escherichia coli strain T-HYM1]
ATGCCGCAACAGATTGAACTGCGCGATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTTAA
ACGCGGGCGTGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCACTGACCTGCGCCGCGACGCTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCCACGCTTACCGCTGCCATAGAAGCGGTGGGGTTGGAAAATGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGTGGCGGTATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCGACTACCGACCTTGATGTGGTAGCGCAGGCGCGCATCCTCGATCTGCTGGAGAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGATGTGGCGGTGA
TGTCACACGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

42

98.425

0.413
  amiE Streptococcus thermophilus LMD-9

42

98.425

0.413
  amiE Streptococcus salivarius strain HSISS4

40.8

98.425

0.402