Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   VLS60_RS01960 Genome accession   NZ_CP141749
Coordinates   301005..301769 (-) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli strain 2023CK-01482     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 296005..306769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VLS60_RS01950 (VLS60_01950) nikR 299795..300196 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  VLS60_RS01955 (VLS60_01955) nikE 300202..301008 (-) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  VLS60_RS01960 (VLS60_01960) amiE 301005..301769 (-) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  VLS60_RS01965 (VLS60_01965) nikC 301769..302602 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  VLS60_RS01970 (VLS60_01970) nikB 302599..303543 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  VLS60_RS01975 (VLS60_01975) nikA 303543..305117 (-) 1575 WP_000953353.1 nickel ABC transporter substrate-binding protein -
  VLS60_RS01980 (VLS60_01980) acpT 305228..305815 (-) 588 WP_000285774.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=837335 VLS60_RS01960 WP_001136236.1 301005..301769(-) (amiE) [Escherichia coli strain 2023CK-01482]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=837335 VLS60_RS01960 WP_001136236.1 301005..301769(-) (amiE) [Escherichia coli strain 2023CK-01482]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAGCAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398