Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   VLW43_RS21185 Genome accession   NZ_CP141736
Coordinates   4306687..4307451 (+) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain 2023CK-01509     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4301687..4312451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VLW43_RS21165 (VLW43_21165) acpT 4302641..4303228 (+) 588 WP_000285774.1 4'-phosphopantetheinyl transferase AcpT -
  VLW43_RS21170 (VLW43_21170) nikA 4303339..4304913 (+) 1575 WP_000953348.1 nickel ABC transporter substrate-binding protein -
  VLW43_RS21175 (VLW43_21175) nikB 4304913..4305857 (+) 945 WP_000947062.1 nickel ABC transporter permease subunit NikB -
  VLW43_RS21180 (VLW43_21180) nikC 4305854..4306687 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  VLW43_RS21185 (VLW43_21185) amiE 4306687..4307451 (+) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  VLW43_RS21190 (VLW43_21190) nikE 4307448..4308254 (+) 807 WP_000173631.1 nickel import ATP-binding protein NikE -
  VLW43_RS21195 (VLW43_21195) nikR 4308260..4308661 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=837314 VLW43_RS21185 WP_001136229.1 4306687..4307451(+) (amiE) [Escherichia coli strain 2023CK-01509]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=837314 VLW43_RS21185 WP_001136229.1 4306687..4307451(+) (amiE) [Escherichia coli strain 2023CK-01509]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
AGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTA
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398