Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   QNH32_RS16640 Genome accession   NZ_CP126088
Coordinates   3167494..3169836 (-) Length   780 a.a.
NCBI ID   WP_076512677.1    Uniprot ID   -
Organism   Priestia flexa strain CCW CN82     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3162494..3174836
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QNH32_RS16615 (QNH32_16615) - 3162558..3162734 (-) 177 WP_035321387.1 hypothetical protein -
  QNH32_RS16620 (QNH32_16620) - 3162816..3163769 (+) 954 WP_239428635.1 N-acetylmuramoyl-L-alanine amidase -
  QNH32_RS16625 (QNH32_16625) dtd 3163817..3164263 (-) 447 WP_025911732.1 D-aminoacyl-tRNA deacylase -
  QNH32_RS16630 (QNH32_16630) - 3164280..3166478 (-) 2199 WP_025911733.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  QNH32_RS16635 (QNH32_16635) - 3166966..3167478 (-) 513 WP_076512678.1 adenine phosphoribosyltransferase -
  QNH32_RS16640 (QNH32_16640) recJ 3167494..3169836 (-) 2343 WP_076512677.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  QNH32_RS16645 (QNH32_16645) - 3169908..3170801 (-) 894 WP_163071332.1 cation diffusion facilitator family transporter -
  QNH32_RS16650 (QNH32_16650) secDF 3170938..3173172 (-) 2235 WP_061784988.1 protein translocase subunit SecDF -
  QNH32_RS16655 (QNH32_16655) - 3173286..3173579 (-) 294 WP_025911741.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 780 a.a.        Molecular weight: 87397.99 Da        Isoelectric Point: 4.7392

>NTDB_id=835196 QNH32_RS16640 WP_076512677.1 3167494..3169836(-) (recJ) [Priestia flexa strain CCW CN82]
MLQSKKRWNVKHIDDPLVDEFAKKLNITPLVASLLINRGIQTVEEASQFLHIEDESFHDPFLLEGMEETVARIRQAIENN
ERIMIYGDYDADGVSSTSVMLSVLGQLGAQVDFYIPNRFTEGYGPNEQAFRQIGDEGYGLVITVDTGISAVKEAKLAAEI
GFDLIITDHHEPGPEIPEAYAIIHPKLPGGGYPFKELAGVGVAFKLAHALLGEAPEHLLELAVIGTIADLVPLVGENRLI
AKKGLRYLKSSTRPGVEALLKVCSVKKEDITEDTIGFALAPRINAVGRLQDANPAVDLLMSTHYEEAIELAKEIDALNKE
RQQIVNEITQEAIDTVESLYPPSENAVLIIEGEGWNAGVVGIVASRLVEKFYRPTIVLSLDHETGLAKGSARSIAGFDLF
ANLSKSRDILPHFGGHPMAAGMTLEAKNVELLRQRLNEQAREVLTEQDFIPITSIDTICKVEDITIAGIEELQTLAPFGM
HNPKPKVLVERVTPTSMRKIGSDKNHLKLVVEDNGLSIDVVGFGLGHVLDEVSPLAKVSLVGELSINEWNNIRKPQLMIE
DVAVSEWQLFDYRNSFDMKRFFQEIFTKEMDIVTFNQQTVQKHAAILPMDQVVEISTDEQASLYNSADKQMILLDLPDSK
ERLNLLFAEGKPARIYAMFVTENDSYFDTIPTREHFKWFYGLLAKRSPFDLKHYKEEIAIHKGWTKDTIDFICQVFFELE
FVTINNGLISLVKNSPKRDLIESVTYRKKQQQIEMEKNFIYSSYAQLKSEFEKLLASADE

Nucleotide


Download         Length: 2343 bp        

>NTDB_id=835196 QNH32_RS16640 WP_076512677.1 3167494..3169836(-) (recJ) [Priestia flexa strain CCW CN82]
ATGCTACAGTCAAAGAAGCGTTGGAATGTAAAACATATAGATGATCCACTTGTTGATGAATTTGCAAAGAAACTCAATAT
TACACCTCTTGTTGCTTCACTGCTTATTAATCGAGGAATTCAAACAGTAGAAGAAGCCTCACAGTTTTTACATATTGAGG
ATGAATCTTTTCATGACCCATTTCTTTTAGAAGGTATGGAAGAAACGGTTGCGCGCATTCGTCAAGCGATTGAAAACAAT
GAACGTATTATGATATATGGGGATTATGATGCTGACGGTGTAAGCAGTACGTCCGTTATGCTTTCTGTTTTAGGTCAACT
CGGAGCGCAAGTTGATTTTTATATACCCAATCGTTTTACAGAAGGCTACGGCCCAAACGAACAGGCATTTAGACAGATTG
GTGATGAAGGATATGGATTAGTTATTACAGTAGATACTGGAATTTCCGCTGTTAAAGAGGCAAAGCTAGCAGCTGAGATA
GGGTTTGATTTAATCATTACAGACCACCATGAACCAGGTCCAGAAATTCCGGAAGCCTATGCGATTATCCATCCTAAACT
TCCAGGTGGAGGCTATCCATTCAAAGAGCTAGCTGGAGTTGGAGTAGCGTTTAAATTAGCTCACGCTTTGCTCGGTGAAG
CTCCTGAACATTTATTGGAGCTAGCTGTCATTGGTACAATTGCTGATTTGGTGCCGCTAGTAGGAGAAAATCGACTAATA
GCGAAAAAGGGCTTACGCTATTTAAAAAGTTCAACTCGACCTGGAGTTGAAGCGCTATTAAAAGTGTGCAGTGTGAAGAA
AGAAGACATTACGGAAGACACAATTGGTTTTGCCCTTGCTCCAAGAATTAATGCTGTAGGTCGTCTTCAAGATGCGAACC
CGGCAGTCGATTTACTTATGTCTACTCATTATGAAGAAGCAATCGAGTTAGCAAAAGAAATTGATGCTTTAAATAAAGAG
CGCCAACAGATTGTAAATGAAATTACGCAAGAAGCAATCGATACTGTAGAAAGCCTATATCCGCCAAGTGAAAATGCAGT
CTTAATTATTGAAGGTGAAGGGTGGAACGCAGGAGTTGTTGGAATCGTAGCTTCTCGGTTGGTTGAAAAGTTCTATCGTC
CAACTATTGTGTTAAGTCTGGATCACGAAACCGGCTTAGCAAAAGGGTCGGCAAGAAGCATTGCTGGATTTGATTTATTT
GCAAACTTATCAAAGAGTCGTGATATTTTACCGCATTTCGGGGGCCACCCGATGGCTGCAGGGATGACGCTTGAAGCGAA
GAATGTTGAGCTACTGCGCCAGCGTTTAAATGAACAAGCGCGAGAAGTGCTAACTGAACAAGACTTCATTCCGATTACCT
CGATTGATACGATATGCAAAGTTGAGGATATTACAATCGCTGGTATTGAAGAGCTCCAAACACTCGCTCCTTTTGGCATG
CATAACCCCAAGCCGAAGGTGCTTGTTGAGCGTGTTACGCCAACATCAATGCGTAAAATCGGCAGTGATAAAAACCATTT
AAAGCTTGTTGTAGAAGATAATGGGCTATCAATTGACGTCGTAGGGTTTGGTTTAGGACATGTATTAGATGAAGTCTCTC
CGTTAGCCAAAGTATCGTTAGTCGGTGAGCTTTCAATTAACGAATGGAATAATATACGCAAGCCACAGCTCATGATTGAA
GATGTAGCAGTGAGTGAATGGCAGCTTTTTGATTATCGTAATTCATTTGATATGAAGAGATTCTTTCAAGAAATTTTCAC
GAAAGAAATGGATATCGTGACGTTTAATCAACAAACCGTTCAAAAGCATGCAGCGATTTTGCCGATGGATCAAGTCGTTG
AGATTTCGACAGATGAACAAGCTAGTCTATATAATTCAGCTGATAAACAAATGATTCTTTTAGATTTACCAGATTCAAAA
GAGAGGTTAAATCTCTTATTTGCTGAAGGAAAGCCGGCTCGAATTTATGCGATGTTCGTAACAGAAAATGATAGTTATTT
TGATACGATTCCGACTCGTGAACATTTTAAGTGGTTTTATGGACTGCTGGCTAAAAGAAGCCCGTTTGATTTAAAACACT
ATAAAGAAGAGATTGCCATTCATAAAGGCTGGACAAAAGATACAATTGATTTTATCTGCCAGGTGTTTTTCGAACTAGAA
TTTGTTACAATAAACAATGGTCTAATTTCGCTAGTGAAAAATAGTCCTAAACGTGATTTAATAGAGTCTGTGACTTATCG
CAAGAAACAGCAACAAATTGAAATGGAAAAAAACTTTATTTATAGTTCATATGCACAGTTAAAAAGTGAGTTTGAGAAGC
TTCTTGCAAGTGCGGATGAATGA

Domains

Predicted by InterproScan.

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(455-561)

DHHA1 domain (DHHA1)

(315-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-769)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

53.718

100

0.537