Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   U2S93_RS27255 Genome accession   NZ_CP140431
Coordinates   5719209..5719634 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain 2023CK-01249     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5714209..5724634
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U2S93_RS27240 (U2S93_27240) pilX 5714773..5715360 (+) 588 WP_003094700.1 type 4a pilus minor pilin PilX -
  U2S93_RS27245 (U2S93_27245) pilY1 5715372..5718863 (+) 3492 WP_022580136.1 type 4a pilus biogenesis protein PilY1 -
  U2S93_RS27250 (U2S93_27250) pilY2 5718865..5719212 (+) 348 WP_003094713.1 type 4a fimbrial biogenesis protein PilY2 -
  U2S93_RS27255 (U2S93_27255) comF 5719209..5719634 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  U2S93_RS27260 (U2S93_27260) ispH 5719681..5720625 (-) 945 WP_003134939.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  U2S93_RS27265 (U2S93_27265) fkpB 5720711..5721151 (-) 441 WP_003094726.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  U2S93_RS27270 (U2S93_27270) lspA 5721144..5721653 (-) 510 WP_003094728.1 signal peptidase II -
  U2S93_RS27275 (U2S93_27275) ileS 5721646..5724477 (-) 2832 WP_003094730.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=834778 U2S93_RS27255 WP_003094721.1 5719209..5719634(+) (comF) [Pseudomonas aeruginosa strain 2023CK-01249]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=834778 U2S93_RS27255 WP_003094721.1 5719209..5719634(+) (comF) [Pseudomonas aeruginosa strain 2023CK-01249]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCAGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTAGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383