Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   VNTUMSATTG_RS16020 Genome accession   NZ_AP024087
Coordinates   3508293..3508727 (+) Length   144 a.a.
NCBI ID   WP_225911751.1    Uniprot ID   -
Organism   Vibrio nigripulchritudo strain TUMSAT-TG-2018     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3503293..3513727
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNTUMSATTG_RS16005 (VNTUMSATTG_31340) pdhR 3505359..3506129 (-) 771 WP_004397664.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  VNTUMSATTG_RS16010 (VNTUMSATTG_31350) ampD 3506442..3506999 (-) 558 WP_200767481.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VNTUMSATTG_RS16015 (VNTUMSATTG_31360) nadC 3507128..3508015 (+) 888 WP_200766159.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VNTUMSATTG_RS16020 (VNTUMSATTG_31370) pilA 3508293..3508727 (+) 435 WP_225911751.1 pilin Machinery gene
  VNTUMSATTG_RS16025 (VNTUMSATTG_31380) pilB 3508727..3510415 (+) 1689 WP_200766161.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VNTUMSATTG_RS16030 (VNTUMSATTG_31390) pilC 3510425..3511657 (+) 1233 WP_200766162.1 type II secretion system F family protein Machinery gene
  VNTUMSATTG_RS16035 (VNTUMSATTG_31400) pilD 3511698..3512567 (+) 870 WP_200766163.1 A24 family peptidase Machinery gene
  VNTUMSATTG_RS16040 (VNTUMSATTG_31410) coaE 3512617..3513222 (+) 606 WP_200766164.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 14650.93 Da        Isoelectric Point: 10.0698

>NTDB_id=83387 VNTUMSATTG_RS16020 WP_225911751.1 3508293..3508727(+) (pilA) [Vibrio nigripulchritudo strain TUMSAT-TG-2018]
MKNKKQQGFTLIELMIVVAIIGVLSAVAIPAYQNHVKKSEAATGLATARALVTNVDMHIQEIGSFPANTAAGFTAVGATT
GMNKLGTLSFTGISGSTGTINFAFGANSSINGASIALAKSVSGWACTFTPAGKIDATEIPKSCK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=83387 VNTUMSATTG_RS16020 WP_225911751.1 3508293..3508727(+) (pilA) [Vibrio nigripulchritudo strain TUMSAT-TG-2018]
ATGAAAAATAAAAAACAACAGGGCTTTACGCTGATAGAGCTAATGATTGTTGTGGCGATTATTGGTGTGTTATCGGCGGT
AGCTATTCCTGCTTACCAGAATCACGTTAAGAAATCTGAAGCGGCTACAGGCTTGGCAACAGCACGTGCATTAGTTACAA
ATGTTGACATGCACATCCAAGAAATTGGCTCTTTCCCAGCAAATACGGCTGCTGGATTTACAGCTGTAGGTGCAACAACG
GGAATGAACAAGCTAGGAACACTTTCCTTTACAGGTATCAGTGGTTCTACTGGTACAATTAACTTTGCATTCGGTGCAAA
CTCATCGATCAATGGCGCATCAATCGCGTTAGCTAAATCAGTTAGTGGATGGGCATGTACTTTCACACCGGCAGGTAAAA
TTGACGCCACAGAGATCCCTAAAAGTTGTAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)

Pilin (bacterial filament) (Pilin)

(38-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

47.619

100

0.486
  pilA Vibrio cholerae strain A1552

47.619

100

0.486
  pilA Vibrio cholerae C6706

47.619

100

0.486
  pilA Vibrio parahaemolyticus RIMD 2210633

49.265

94.444

0.465
  pilA Pseudomonas aeruginosa PAK

38.411

100

0.403
  pilA/pilA1 Eikenella corrodens VA1

37.255

100

0.396
  comP Acinetobacter baylyi ADP1

34.591

100

0.382
  pilA Acinetobacter baumannii strain A118

36.806

100

0.368
  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.552

100

0.368
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.324

98.611

0.368

Multiple sequence alignment