Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   U0530_RS01935 Genome accession   NZ_CP139853
Coordinates   380055..380522 (+) Length   155 a.a.
NCBI ID   WP_000340159.1    Uniprot ID   -
Organism   Escherichia coli strain Nord122     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 375055..385522
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0530_RS01890 (U0530_01885) rplP 375165..375575 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  U0530_RS01895 (U0530_01890) rpsC 375588..376289 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  U0530_RS01900 (U0530_01895) rplV 376307..376639 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  U0530_RS01905 (U0530_01900) rpsS 376654..376932 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  U0530_RS01910 (U0530_01905) rplB 376949..377770 (-) 822 WP_322436417.1 50S ribosomal protein L2 -
  U0530_RS01915 (U0530_01910) rplW 377788..378090 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  U0530_RS01920 (U0530_01915) rplD 378087..378692 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  U0530_RS01925 (U0530_01920) rplC 378703..379332 (-) 630 WP_322436418.1 50S ribosomal protein L3 -
  U0530_RS01930 (U0530_01925) rpsJ 379365..379676 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  U0530_RS01935 (U0530_01930) pilD 380055..380522 (+) 468 WP_000340159.1 prepilin peptidase Machinery gene
  U0530_RS01940 (U0530_01935) bfr 380519..380995 (-) 477 WP_000675515.1 bacterioferritin -
  U0530_RS01945 (U0530_01940) bfd 381068..381262 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  U0530_RS01950 (U0530_01945) tuf 381445..382629 (-) 1185 WP_000031783.1 elongation factor Tu -
  U0530_RS01955 (U0530_01950) fusA 382700..384814 (-) 2115 WP_000124703.1 elongation factor G -
  U0530_RS01960 (U0530_01955) rpsG 384911..385381 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17034.17 Da        Isoelectric Point: 8.1255

>NTDB_id=831952 U0530_RS01935 WP_000340159.1 380055..380522(+) (pilD) [Escherichia coli strain Nord122]
MDATLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=831952 U0530_RS01935 WP_000340159.1 380055..380522(+) (pilD) [Escherichia coli strain Nord122]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCAGCCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACGTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAAGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACATGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGCTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(11-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.882

81.935

0.368