Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   U0532_RS13810 Genome accession   NZ_CP139808
Coordinates   2862531..2863295 (-) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain FLI21     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 2857531..2868295
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0532_RS13800 (U0532_13800) nikR 2861321..2861722 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  U0532_RS13805 (U0532_13805) nikE 2861728..2862534 (-) 807 WP_000173631.1 nickel import ATP-binding protein NikE -
  U0532_RS13810 (U0532_13810) amiE 2862531..2863295 (-) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  U0532_RS13815 (U0532_13815) nikC 2863295..2864128 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  U0532_RS13820 (U0532_13820) nikB 2864125..2865069 (-) 945 WP_000947062.1 nickel ABC transporter permease subunit NikB -
  U0532_RS13825 (U0532_13825) nikA 2865069..2866643 (-) 1575 WP_072731500.1 nickel ABC transporter substrate-binding protein -
  U0532_RS13830 (U0532_13830) acpT 2866754..2867341 (-) 588 WP_000285791.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=831672 U0532_RS13810 WP_001136229.1 2862531..2863295(-) (amiE) [Escherichia coli strain FLI21]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=831672 U0532_RS13810 WP_001136229.1 2862531..2863295(-) (amiE) [Escherichia coli strain FLI21]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398