Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   TT393_RS13395 Genome accession   NZ_CP139803
Coordinates   2727958..2728722 (+) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli strain HN257     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 2722958..2733722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TT393_RS13375 (TT393_13375) acpT 2723912..2724499 (+) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -
  TT393_RS13380 (TT393_13380) nikA 2724610..2726184 (+) 1575 WP_000953353.1 nickel ABC transporter substrate-binding protein -
  TT393_RS13385 (TT393_13385) nikB 2726184..2727128 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  TT393_RS13390 (TT393_13390) nikC 2727125..2727958 (+) 834 WP_001008968.1 nickel ABC transporter permease subunit NikC -
  TT393_RS13395 (TT393_13395) amiE 2727958..2728722 (+) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  TT393_RS13400 (TT393_13400) nikE 2728719..2729525 (+) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  TT393_RS13405 (TT393_13405) nikR 2729531..2729932 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  TT393_RS13410 (TT393_13410) - 2730052..2730411 (-) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  TT393_RS13415 (TT393_13415) - 2730408..2730638 (-) 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin -
  TT393_RS13420 (TT393_13420) yhhJ 2730742..2731866 (-) 1125 WP_001314210.1 ABC transporter permease -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=831592 TT393_RS13395 WP_001136236.1 2727958..2728722(+) (amiE) [Escherichia coli strain HN257]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=831592 TT393_RS13395 WP_001136236.1 2727958..2728722(+) (amiE) [Escherichia coli strain HN257]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGAGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398