Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   SPM78_RS21265 Genome accession   NZ_CP139424
Coordinates   4494771..4495196 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain 136790     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4489771..4500196
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPM78_RS21250 (SPM78_21250) pilX 4490335..4490922 (+) 588 WP_003094700.1 type 4a pilus minor pilin PilX -
  SPM78_RS21255 (SPM78_21255) pilY1 4490934..4494425 (+) 3492 WP_023096128.1 type 4a pilus biogenesis protein PilY1 -
  SPM78_RS21260 (SPM78_21260) pilY2 4494427..4494774 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  SPM78_RS21265 (SPM78_21265) comF 4494771..4495196 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  SPM78_RS21270 (SPM78_21270) ispH 4495243..4496187 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  SPM78_RS21275 (SPM78_21275) fkpB 4496273..4496713 (-) 441 WP_003094726.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  SPM78_RS21280 (SPM78_21280) lspA 4496706..4497215 (-) 510 WP_003094728.1 signal peptidase II -
  SPM78_RS21285 (SPM78_21285) ileS 4497208..4500039 (-) 2832 WP_003094730.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=830352 SPM78_RS21265 WP_003094721.1 4494771..4495196(+) (comF) [Pseudomonas aeruginosa strain 136790]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=830352 SPM78_RS21265 WP_003094721.1 4494771..4495196(+) (comF) [Pseudomonas aeruginosa strain 136790]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383