Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   SHK14_RS01190 Genome accession   NZ_CP139337
Coordinates   261408..262172 (-) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain YZ22MPE54     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 256408..267172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SHK14_RS01180 nikR 260198..260599 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  SHK14_RS01185 nikE 260605..261411 (-) 807 WP_000173631.1 nickel import ATP-binding protein NikE -
  SHK14_RS01190 amiE 261408..262172 (-) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  SHK14_RS01195 nikC 262172..263005 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  SHK14_RS01200 nikB 263002..263946 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  SHK14_RS01205 nikA 263946..265520 (-) 1575 WP_000953361.1 nickel ABC transporter substrate-binding protein -
  SHK14_RS01210 acpT 265631..266218 (-) 588 WP_000285774.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=830055 SHK14_RS01190 WP_001136229.1 261408..262172(-) (amiE) [Escherichia coli strain YZ22MPE54]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=830055 SHK14_RS01190 WP_001136229.1 261408..262172(-) (amiE) [Escherichia coli strain YZ22MPE54]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398