Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   SE692_RS21570 Genome accession   NZ_CP138423
Coordinates   4291658..4292422 (+) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain 30EVA1     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4286658..4297422
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SE692_RS21550 acpT 4287612..4288199 (+) 588 WP_000285791.1 4'-phosphopantetheinyl transferase AcpT -
  SE692_RS21555 nikA 4288310..4289884 (+) 1575 WP_112920763.1 nickel ABC transporter substrate-binding protein -
  SE692_RS21560 nikB 4289884..4290828 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  SE692_RS21565 nikC 4290825..4291658 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  SE692_RS21570 amiE 4291658..4292422 (+) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  SE692_RS21575 nikE 4292419..4293225 (+) 807 WP_000173631.1 nickel import ATP-binding protein NikE -
  SE692_RS21580 nikR 4293231..4293632 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=826322 SE692_RS21570 WP_001136229.1 4291658..4292422(+) (amiE) [Escherichia coli strain 30EVA1]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=826322 SE692_RS21570 WP_001136229.1 4291658..4292422(+) (amiE) [Escherichia coli strain 30EVA1]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398