Detailed information NTG0823705

Bioinformatically predicted

Overview

• Name: addB
• Type: Machinery gene
• Locus tag: QHF92_RS05980
• Genome accession: NZ_CP123994
• Coordinates: 1245950..1249450 (+)
• Length: 1166 a.a.
• NCBI ID: WP_060398327.1
• Uniprot ID: -
• Organism: Bacillus inaquosorum strain M3
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1240950..1254450

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QHF92_RS05960 (QHF92_05960)	-	1241804..1243006 (-)	1203	WP_060398324.1	MFS transporter	-
QHF92_RS05965 (QHF92_05965)	-	1243120..1244847 (+)	1728	WP_060398325.1	ABC transporter substrate-binding protein	-
QHF92_RS05970 (QHF92_05970)	-	1244884..1245213 (-)	330	WP_060398326.1	four-helix bundle copper-binding protein	-
QHF92_RS05975 (QHF92_05975)	-	1245330..1245767 (-)	438	WP_044427329.1	ferritin family protein	-
QHF92_RS05980 (QHF92_05980)	addB	1245950..1249450 (+)	3501	WP_060398327.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
QHF92_RS05985 (QHF92_05985)	addA	1249437..1253141 (+)	3705	WP_060398328.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
QHF92_RS05990 (QHF92_05990)	sbcD	1253213..1254388 (+)	1176	WP_060398329.1	exonuclease subunit SbcD	-

Sequence

Protein

• Length: 1166 a.a.
• Molecular weight: 134654.76 Da
• Isoelectric Point: 5.6870

>NTDB_id=823705 QHF92_RS05980 WP_060398327.1 1245950..1249450(+) (addB) [Bacillus inaquosorum strain M3]
MGAEFLVGRSGSGKTKLIIDSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDMGGMIRAQVFSFSRLAWRVLQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMVESGTASEYRGERILSE
KLHDLSILYQQMEKSLAGQYLHSEDYLTLLAEHVPLAEDVKGAHIYVDGFYQFTPQEFRVLEQLMVHAEHITFSLTADKP
SYEREPHELELFRMTGKTYYRLHQKAKELNLDITYKELSGTERHTRTPELAHLEAQYEARPAVPYAEKQEAFTVMQAANR
RAELEGIAREIHALVREKGYRYKDVAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSLDVLKGNWRY
EAVFRCVKTELLFPLKEPKTKVREQVDQLENYCIAYGIKGDRWTKGDRFHYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVQEKAEALYRYLEETDVPLKLDQERQRAEEDGRIIEAQQHQQAWDAVIQLLEEFVEMMGDDEI
SLDLFQQMMEAGAESLTFSLIPPALDQVFVGNMDLSRMYGTSCTFVLGANDGVLPARPDENGVLSDDDREWLKTVGVELS
SGGRERLLDEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPSIVIKRLEELFPQHKERLLTNEPEQVSDEEQLMYVVNKS
VAQSFTASQLRLWTREYDISDVWWSTYNVLMSEPDRLQSKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKMDWRDLTKEQCELFSHDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFKLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEAIRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFELLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRPLKAEKDKTILEWIKKEADGNEHS

Nucleotide

• Length: 3501 bp

>NTDB_id=823705 QHF92_RS05980 WP_060398327.1 1245950..1249450(+) (addB) [Bacillus inaquosorum strain M3]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCGACAGCATTCAGGATGAACTGCG
CCGGGCTCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCTGATATGGGCGGAATGATACGCGCTCAAGTGTTCAGCTTTTCACGATTGGCCTGGCGCGTCCTCCAGCATACGGGA
GGCATGAGCAGGCCGTTTCTTACGAGCACAGGCGTACAGATGCTCCTGCGGAAGCTCATTGAGGAGCATAAACAGGAGTT
TAAAGTCTATCAAAAAGCGAGTGACAAAAGCGGGTTTACCGCACAGGTAGAACGGATGCTGACAGAGTTTAAGCGCTACT
GTCTGGAACCGGAAGACATCCGCCGGATGGTGGAAAGCGGAACGGCTTCCGAGTATCGCGGAGAACGTATTTTATCTGAG
AAGCTTCATGATTTATCGATTTTATATCAACAGATGGAAAAAAGCCTTGCAGGTCAATACCTTCACTCTGAGGATTATTT
AACATTGCTGGCAGAGCATGTTCCGCTTGCAGAAGATGTAAAAGGCGCGCATATCTATGTGGACGGCTTTTATCAGTTTA
CACCGCAGGAATTCAGGGTGTTGGAGCAGCTTATGGTTCATGCAGAGCACATCACGTTTTCGCTGACAGCGGACAAGCCG
TCATATGAGCGGGAGCCGCATGAACTGGAATTGTTCAGAATGACGGGAAAAACCTATTACCGCCTGCACCAAAAGGCGAA
GGAACTGAATCTGGACATTACGTATAAAGAGCTAAGCGGGACTGAACGGCATACGCGCACACCGGAATTGGCACATTTAG
AGGCGCAGTATGAAGCGCGTCCAGCCGTTCCGTACGCAGAAAAACAAGAGGCCTTTACTGTGATGCAGGCCGCAAACAGA
CGAGCTGAGCTGGAAGGCATTGCCCGGGAAATTCACGCTCTTGTCAGAGAGAAGGGCTATCGCTATAAGGATGTAGCGAT
TCTTGCGCGCCAGCCGGAAGACTACAAGGATATGGTGAAGGAAGTTTTCGCAGATTACGAGATTCCTTATTTCATTGACG
GAAAAGCATCAATGCTGAACCATCCGTTAATCGAATTTATCCGGTCGAGCCTTGATGTTTTGAAAGGGAATTGGCGTTAT
GAAGCAGTGTTTCGCTGCGTGAAAACCGAACTGCTATTCCCGCTTAAAGAGCCGAAGACTAAGGTGAGAGAACAGGTCGA
TCAGCTTGAAAATTACTGTATCGCCTACGGGATCAAAGGTGACCGCTGGACAAAGGGCGACCGTTTCCATTACAGACGTT
TTGTGTCATTGGATGATGATTTTGCGCAGACCGACCAGGAAATCGAAATGGAAAACATGCTGAATGACACACGCGACTGG
ATTGTTCCTCCGCTTTTTCAGCTCCAGAAACGAATGAAAAAAGCAAAGACGGTTCAGGAGAAGGCGGAGGCGCTCTACCG
CTATCTAGAAGAGACGGATGTGCCGCTGAAGCTGGATCAGGAAAGACAGCGTGCAGAGGAAGACGGCAGAATCATTGAAG
CACAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTGCTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATT
TCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCAGAGTCGCTTACATTTTCTCTTATTCCGCCTGCGCTTGACCA
GGTGTTTGTCGGCAATATGGATTTGTCCAGAATGTATGGCACCTCCTGTACCTTTGTGCTGGGGGCAAACGACGGCGTTC
TGCCGGCGCGCCCAGATGAAAACGGAGTGCTGTCGGATGATGACCGGGAATGGCTGAAAACGGTTGGGGTTGAGCTTTCC
TCAGGCGGGCGGGAGCGTCTGCTTGATGAGCATTTCCTCATCTATATGGCGTTTTCCAGTCCGTCTGACCGGCTGTACGT
ATCGTATCCGATTGCTGATGCGGAAGGAAAAACGCTTCTGCCGTCGATTGTCATTAAGCGGCTGGAAGAACTGTTTCCGC
AGCATAAGGAGCGCCTGTTGACAAATGAACCTGAGCAGGTCAGCGATGAGGAACAGCTGATGTATGTCGTCAATAAAAGC
GTGGCACAGTCCTTTACCGCGAGCCAGCTCAGGTTATGGACTCGGGAATACGACATCAGCGACGTCTGGTGGAGCACGTA
CAATGTGTTGATGAGTGAGCCGGACAGGCTGCAATCGAAAAAGTTATTCTCAAGCCTGTTTTTCCGGAATGAAGTGAAGC
AGCTTGAACGCAGCGTGTCGAGACAGCTCTATGGTGAACGCATTCAGGGCAGTGTATCGAGAATGGAAACCTTTAACGCG
TGCCCGTTCTCCCATTTTGCGTCACACGGGCTGCATCTGAAGGAACGGCAATTTTTCAAGCTCGAAGCACCGGATATCGG
CCAGCTGTTTCATTCCAGCTTAAAGCTGATTTCAGACCGGCTGCGCGAGCAAAAAATGGATTGGCGCGATTTAACGAAAG
AGCAGTGCGAGCTGTTCTCCCATGATGCGGTAGAGCGTCTGGCGCCGAAACTGCAAAAGGAAATTTTGCTCAGCTCAAAC
CGGCATTATTATGTGAAGGAAAAACTGCAAAAAATTGTGACACGCGTGTCCGGCATTTTAAGCGAGCATGCGAAAGCGAG
CGGTTTCGTGCCGATCGGGCTTGAACTGGGCTTTGGAGGAAAAGGGCCGCTTCCGCCGCTGACCTTTAAGCTGAAAAACG
GCTGCACGATGGAGCTCGTCGGGCGAATTGACCGCGTAGATAAAGCGGAAAGCTCAAAAGGCCTGCTTCTAAGAATTGTC
GATTATAAATCAAGCGACAAAGGCCTTGACTTAGCGGAAGTATATTACGGACTGGCGCTGCAAATGCTGACGTACCTTGA
TTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGAGCGACGCCGGCCGGAGTGCTGTATTTCCATATTCATGACC
CGATGATTCAATCTAACCTCCCGCTTGGGCTTGACGAGATCGAACAGGAGATCTTTAAGAAATTTAAGATGAAGGGCCTG
CTCCTCGGCGATCAGGAAGCGATTCGCCTCATGGATACGACTCTTCAAGAGGGACGGTCAAATATCATCAACGCCGGCTT
GAAAAAAGACGGCTCTCTCAGATCGGACTCGGCGGCAGTCGGCGAAAAGGAATTTGAGCTGCTGACGAAGCATGTGCGCC
GCACCTTCCAAGAAGCAGGCGAACAAATCACCGACGGACGCGTATCGATTGAGCCTTATAAAATGAAGAACAAGACACCG
TGCACATACTGTGCGTTCAAATCAGTATGCCAATTTGATGAATCATTAGAAGAAAACGAGTATCGCCCGTTAAAGGCTGA
AAAGGACAAGACGATACTTGAGTGGATAAAAAAGGAGGCGGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (306-615)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (790-1131)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addB	Bacillus subtilis subsp. subtilis str. 168	97.77	100	0.978