Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   R6I05_RS01200 Genome accession   NZ_CP137705
Coordinates   261587..262351 (-) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli isolate FELIX_MS724     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 256587..267351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R6I05_RS01175 (R6I05_01175) yhhJ 258429..259553 (+) 1125 WP_001488398.1 ABC transporter permease -
  R6I05_RS01180 (R6I05_01180) - 259626..259901 (+) 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin -
  R6I05_RS01185 (R6I05_01185) - 259898..260257 (+) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  R6I05_RS01190 (R6I05_01190) nikR 260377..260778 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  R6I05_RS01195 (R6I05_01195) nikE 260784..261590 (-) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  R6I05_RS01200 (R6I05_01200) amiE 261587..262351 (-) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  R6I05_RS01205 (R6I05_01205) nikC 262351..263184 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  R6I05_RS01210 (R6I05_01210) nikB 263181..264125 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  R6I05_RS01215 (R6I05_01215) nikA 264125..265699 (-) 1575 WP_000953353.1 nickel ABC transporter substrate-binding protein -
  R6I05_RS01220 (R6I05_01220) acpT 265810..266397 (-) 588 WP_000285774.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=823218 R6I05_RS01200 WP_001136236.1 261587..262351(-) (amiE) [Escherichia coli isolate FELIX_MS724]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=823218 R6I05_RS01200 WP_001136236.1 261587..262351(-) (amiE) [Escherichia coli isolate FELIX_MS724]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398