Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   R6I08_RS01215 Genome accession   NZ_CP137696
Coordinates   262482..263246 (-) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli isolate FELIX_MS533     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 257482..268246
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R6I08_RS01205 (R6I08_01205) nikR 261272..261673 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  R6I08_RS01210 (R6I08_01210) nikE 261679..262485 (-) 807 WP_000173631.1 nickel import ATP-binding protein NikE -
  R6I08_RS01215 (R6I08_01215) amiE 262482..263246 (-) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  R6I08_RS01220 (R6I08_01220) nikC 263246..264079 (-) 834 WP_097496312.1 nickel ABC transporter permease subunit NikC -
  R6I08_RS01225 (R6I08_01225) nikB 264076..265020 (-) 945 WP_000947064.1 nickel ABC transporter permease subunit NikB -
  R6I08_RS01230 (R6I08_01230) nikA 265020..266594 (-) 1575 WP_000953361.1 nickel ABC transporter substrate-binding protein -
  R6I08_RS01235 (R6I08_01235) acpT 266705..267292 (-) 588 WP_000285774.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=823137 R6I08_RS01215 WP_001136229.1 262482..263246(-) (amiE) [Escherichia coli isolate FELIX_MS533]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=823137 R6I08_RS01215 WP_001136229.1 262482..263246(-) (amiE) [Escherichia coli isolate FELIX_MS533]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398