Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   R6U80_RS09845 Genome accession   NZ_CP137623
Coordinates   2029321..2030100 (-) Length   259 a.a.
NCBI ID   WP_050428201.1    Uniprot ID   -
Organism   Lactococcus lactis strain NCK401     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2024321..2035100
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R6U80_RS09825 (R6U80_09825) pflA 2025046..2025840 (-) 795 WP_003130318.1 pyruvate formate-lyase-activating protein -
  R6U80_RS09830 (R6U80_09830) - 2026046..2027395 (-) 1350 WP_058223249.1 hemolysin family protein -
  R6U80_RS09835 (R6U80_09835) - 2027517..2028632 (-) 1116 WP_010906150.1 AI-2E family transporter -
  R6U80_RS09840 (R6U80_09840) - 2028781..2029230 (+) 450 WP_058223250.1 GNAT family N-acetyltransferase -
  R6U80_RS09845 (R6U80_09845) endA 2029321..2030100 (-) 780 WP_050428201.1 DNA/RNA non-specific endonuclease Machinery gene
  R6U80_RS09850 (R6U80_09850) - 2030294..2031190 (-) 897 WP_039115881.1 peptidoglycan amidohydrolase family protein -
  R6U80_RS09855 (R6U80_09855) - 2031302..2031556 (-) 255 WP_023189062.1 phage holin -
  R6U80_RS09860 (R6U80_09860) - 2031546..2031836 (-) 291 WP_124175219.1 hypothetical protein -
  R6U80_RS09865 (R6U80_09865) - 2032053..2032346 (+) 294 WP_313791928.1 hypothetical protein -
  R6U80_RS09870 (R6U80_09870) - 2032376..2034616 (-) 2241 WP_098397295.1 glycoside hydrolase domain-containing protein -
  R6U80_RS09875 (R6U80_09875) - 2034716..2035063 (-) 348 WP_023189066.1 DUF722 domain-containing protein -

Sequence

Protein


Download         Length: 259 a.a.        Molecular weight: 29129.05 Da        Isoelectric Point: 9.0583

>NTDB_id=822692 R6U80_RS09845 WP_050428201.1 2029321..2030100(-) (endA) [Lactococcus lactis strain NCK401]
MKNKKIIPLLIVSIFILGGCANSQSDMDKSNHKWSQKDKTPSEELAKSVLTSSIKEQLKTDNIKFDHGSFIVNNNNNDLA
IINVVDPYAKNEIDIQGRPYLGEAVLPKSIQNNEKDNKDEKVIPQFGWGQKSNLKGFYKKAYTKGFLLNSKLIGGVKDFN
DSGSNMENIFTQTVWASEANDSSSKGQKYFENMIHKVQDKNMIIKYRVKALYDGSNLVPSGTEIEAKSDDFSLEIHVFIP
NVQPNLKVDYKTGQVTEVK

Nucleotide


Download         Length: 780 bp        

>NTDB_id=822692 R6U80_RS09845 WP_050428201.1 2029321..2030100(-) (endA) [Lactococcus lactis strain NCK401]
ATGAAGAACAAAAAAATTATCCCATTACTGATTGTCAGCATTTTTATCTTAGGGGGCTGTGCTAACAGTCAATCTGATAT
GGATAAAAGTAATCATAAATGGAGTCAAAAAGATAAGACACCCTCAGAAGAACTCGCAAAAAGTGTTCTGACTTCTTCAA
TAAAAGAACAATTAAAAACAGATAACATAAAATTTGATCACGGTTCATTTATCGTTAATAATAATAACAATGATTTAGCA
ATTATTAATGTTGTAGACCCATACGCTAAGAATGAAATAGATATTCAAGGGCGTCCTTATTTGGGTGAAGCAGTTCTCCC
AAAATCTATTCAAAATAATGAAAAGGATAATAAAGATGAAAAAGTGATTCCTCAATTTGGTTGGGGACAAAAATCTAACT
TGAAAGGCTTCTATAAAAAAGCTTATACTAAAGGTTTTTTGCTAAATAGTAAACTAATAGGTGGCGTTAAAGATTTCAAT
GATAGCGGGTCAAATATGGAAAATATATTTACTCAAACGGTTTGGGCGAGTGAAGCTAATGATTCAAGTTCCAAGGGACA
AAAATATTTTGAAAATATGATTCATAAAGTTCAAGATAAGAATATGATCATCAAGTATAGAGTGAAAGCACTCTATGATG
GAAGTAACCTTGTCCCATCAGGAACTGAAATAGAAGCAAAATCTGATGATTTTTCACTCGAGATACATGTTTTTATTCCA
AATGTTCAACCAAATCTTAAAGTAGATTATAAAACAGGTCAGGTCACTGAAGTCAAATAA

Domains

Predicted by InterProScan.

DNA/RNA non-specific endonuclease (Endonuclea_NS_2)

(92-229)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

42.174

88.803

0.375
  endA Streptococcus pneumoniae D39

42.174

88.803

0.375
  endA Streptococcus pneumoniae R6

42.174

88.803

0.375
  endA Streptococcus pneumoniae TIGR4

42.174

88.803

0.375