Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   R5024_RS25685 Genome accession   NZ_CP137510
Coordinates   5468538..5468963 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain HPA0310     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5463538..5473963
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5024_RS25670 (R5024_25670) pilX 5464102..5464689 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  R5024_RS25675 (R5024_25675) pilY1 5464701..5468192 (+) 3492 WP_003123397.1 type 4a pilus biogenesis protein PilY1 -
  R5024_RS25680 (R5024_25680) pilY2 5468194..5468541 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  R5024_RS25685 (R5024_25685) comF 5468538..5468963 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  R5024_RS25690 (R5024_25690) ispH 5469010..5469954 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  R5024_RS25695 (R5024_25695) fkpB 5470040..5470480 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  R5024_RS25700 (R5024_25700) lspA 5470473..5470982 (-) 510 WP_003094728.1 signal peptidase II -
  R5024_RS25705 (R5024_25705) ileS 5470975..5473806 (-) 2832 WP_003102617.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=821694 R5024_RS25685 WP_003094721.1 5468538..5468963(+) (comF) [Pseudomonas aeruginosa strain HPA0310]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=821694 R5024_RS25685 WP_003094721.1 5468538..5468963(+) (comF) [Pseudomonas aeruginosa strain HPA0310]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCAGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGGCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383