Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   R5015_RS10065 Genome accession   NZ_CP137505
Coordinates   2068036..2068632 (+) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain HPA0044     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2063036..2073632
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5015_RS10055 (R5015_10055) - 2064316..2066787 (-) 2472 WP_003095841.1 penicillin-binding protein 1A -
  R5015_RS10060 (R5015_10060) pilM 2066972..2068036 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  R5015_RS10065 (R5015_10065) pilN 2068036..2068632 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  R5015_RS10070 (R5015_10070) pilO 2068629..2069252 (+) 624 WP_003095838.1 type 4a pilus biogenesis protein PilO Machinery gene
  R5015_RS10075 (R5015_10075) pilP 2069249..2069773 (+) 525 WP_317950573.1 type 4a pilus biogenesis lipoprotein PilP -
  R5015_RS10080 (R5015_10080) pilQ 2069827..2071971 (+) 2145 WP_317950575.1 type 4a pilus secretin PilQ Machinery gene
  R5015_RS10085 (R5015_10085) aroK 2071983..2072501 (+) 519 WP_003095833.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=821459 R5015_RS10065 WP_003095839.1 2068036..2068632(+) (pilN) [Pseudomonas aeruginosa strain HPA0044]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=821459 R5015_RS10065 WP_003095839.1 2068036..2068632(+) (pilN) [Pseudomonas aeruginosa strain HPA0044]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCGGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGATTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGTCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATTGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGTTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369