Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   R5015_RS08230 Genome accession   NZ_CP137505
Coordinates   1732329..1734113 (-) Length   594 a.a.
NCBI ID   WP_003096276.1    Uniprot ID   A0A0H2ZIS8
Organism   Pseudomonas aeruginosa strain HPA0044     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1727329..1739113
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5015_RS08210 (R5015_08210) gcvH 1727721..1728110 (+) 390 WP_003107397.1 glycine cleavage system protein GcvH -
  R5015_RS08215 (R5015_08215) gcvP 1728284..1731159 (+) 2876 Protein_1626 aminomethyl-transferring glycine dehydrogenase -
  R5015_RS08220 (R5015_08220) - 1731194..1731523 (-) 330 WP_003096280.1 DUF2388 domain-containing protein -
  R5015_RS08225 (R5015_08225) - 1731655..1732107 (-) 453 WP_003096278.1 NfeD family protein -
  R5015_RS08230 (R5015_08230) pilB 1732329..1734113 (-) 1785 WP_003096276.1 GspE/PulE family protein Machinery gene
  R5015_RS08235 (R5015_08235) - 1734151..1735515 (-) 1365 WP_003096273.1 inorganic triphosphatase -
  R5015_RS08240 (R5015_08240) - 1735629..1736306 (+) 678 WP_003096272.1 TIGR00153 family protein -
  R5015_RS08245 (R5015_08245) - 1736353..1737621 (+) 1269 WP_003096269.1 inorganic phosphate transporter -
  R5015_RS08250 (R5015_08250) argE 1737721..1738875 (+) 1155 WP_003096268.1 acetylornithine deacetylase -

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 65981.61 Da        Isoelectric Point: 6.5096

>NTDB_id=821453 R5015_RS08230 WP_003096276.1 1732329..1734113(-) (pilB) [Pseudomonas aeruginosa strain HPA0044]
MSVFATAAQDRLLDLGDLLRELVAQGRVGQEQAEQCLAIRRSAVRNQQHPLEFLAAQQVEDLKRPGRKLDLETLTQWLAE
YAGQPYLRIDPLKIDVAAVTPLMSYAFAQRHGILAVALSPEEVTIASAQPFVQGWESNLVHVLKRPIKRVVANPADIQRY
TVEFFRLARSVSGATSPDQKISGVGNFEQLLNLGAADQEPDANDAHIVTIVDWLFQYAFQQRASDIHIEPRREQGTVRFR
IDGVLHNVYQFPPQVTMAVVSRLKSLGRMNVAEKRKPQDGRVKTKTPDGAEVELRLSTLPTAFGEKMVMRIFDPEVLLKS
FDQLGFSADDLRRWQSMTSQPNGIILVTGPTGSGKTTTLYTTLKQLATDEVNVCTIEDPIEMIEPSFNQMQVQHNIDLTF
ASGVRALLRQDPDIIMVGEIRDLETAEMAIQAALTGHLVLSTLHTNDSPTAITRLLELGVPYYLIRATVLGVMAQRLVRT
LCPHCKAPMQLADSDWHELTRPWSAPPPSGAHQAVGCLECRDTGYRGRAGVYEIMLLSDSLKELITADTDLVALRRQAYK
EGTRSLRLSGAQKVAAGISTLEEVLRVTPQSEQK

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=821453 R5015_RS08230 WP_003096276.1 1732329..1734113(-) (pilB) [Pseudomonas aeruginosa strain HPA0044]
ATGTCCGTGTTCGCCACCGCCGCCCAGGACCGCCTGCTGGACCTCGGCGACCTGCTCCGCGAGCTGGTCGCCCAGGGCCG
GGTCGGGCAGGAGCAGGCCGAACAGTGCCTGGCGATCCGCCGCAGCGCGGTGCGCAACCAGCAGCATCCGCTGGAGTTCC
TCGCCGCGCAGCAGGTCGAGGATCTCAAGCGCCCCGGACGCAAGCTCGACCTGGAGACCCTCACCCAGTGGCTGGCCGAG
TACGCCGGCCAGCCCTACCTGCGCATCGACCCGCTGAAGATCGACGTCGCCGCGGTGACTCCGCTGATGTCCTACGCCTT
CGCCCAGCGCCATGGCATCCTCGCCGTCGCGCTGAGTCCGGAGGAAGTCACCATCGCCAGCGCCCAGCCCTTCGTACAGG
GCTGGGAAAGCAACCTGGTGCATGTGCTCAAGCGACCGATCAAGCGGGTGGTGGCGAACCCGGCGGACATCCAGCGCTAC
ACCGTCGAGTTCTTCCGCCTGGCCCGCTCGGTCAGCGGCGCCACCTCGCCGGACCAGAAGATCAGCGGCGTCGGCAACTT
CGAGCAGTTGCTCAACCTCGGCGCCGCCGACCAGGAGCCGGACGCCAACGACGCGCACATCGTCACCATCGTCGACTGGC
TGTTCCAGTACGCCTTCCAGCAGCGCGCCAGCGACATCCACATCGAGCCGCGCCGCGAGCAGGGCACCGTGCGCTTCCGT
ATCGACGGCGTGCTGCACAACGTCTACCAGTTCCCGCCGCAGGTGACCATGGCGGTGGTCAGCCGCCTGAAGTCGCTGGG
ACGGATGAACGTGGCGGAGAAACGCAAGCCGCAGGACGGCCGGGTCAAGACCAAGACCCCGGACGGCGCCGAGGTGGAGC
TGCGCCTGTCGACCCTGCCCACCGCCTTCGGCGAGAAGATGGTGATGCGCATCTTCGACCCCGAGGTGCTGCTCAAGAGC
TTCGACCAGCTCGGCTTCTCCGCCGACGACCTGCGCCGCTGGCAGAGCATGACCAGCCAGCCCAACGGCATCATCCTGGT
CACCGGCCCCACCGGCTCGGGCAAGACCACCACGCTCTACACCACGCTCAAGCAACTGGCCACCGACGAGGTGAACGTCT
GCACCATCGAGGACCCGATCGAGATGATCGAGCCCTCGTTCAACCAGATGCAGGTCCAGCACAACATCGACCTGACCTTC
GCCAGCGGCGTACGCGCCCTGCTGCGGCAGGACCCGGACATCATCATGGTCGGCGAGATCCGCGACCTGGAGACCGCCGA
GATGGCGATCCAGGCGGCGCTCACCGGGCACCTGGTGCTCTCCACCCTGCACACCAACGACTCGCCCACGGCGATCACCC
GCCTGCTCGAACTGGGCGTGCCCTACTACCTGATCCGCGCCACGGTGCTCGGGGTCATGGCCCAGCGCCTGGTGCGCACA
CTCTGCCCGCACTGCAAGGCGCCGATGCAACTGGCGGACAGCGACTGGCACGAACTGACCCGCCCCTGGAGCGCCCCGCC
ACCCAGCGGCGCGCACCAGGCCGTGGGTTGCCTGGAGTGCCGCGACACCGGCTATCGCGGGCGCGCCGGCGTCTATGAAA
TCATGCTGCTCAGCGATAGCCTGAAAGAACTGATCACCGCCGACACCGACCTGGTCGCCCTGCGCCGCCAGGCGTACAAA
GAAGGCACCCGCAGCCTGCGCCTGTCCGGCGCGCAGAAGGTCGCCGCGGGTATCAGTACGCTGGAGGAAGTACTGCGGGT
GACCCCGCAGAGCGAACAGAAGTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(216-480)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(69-167)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZIS8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.682

90.067

0.375